• Immunohistochemistry analysis of paraffin-embedded mouse brain using PTPRE antibody (STJ29289) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using PTPRE antibody (STJ29289) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PTPRE antibody (STJ29289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PTPRE antibody (20-200) (STJ29289)

SKU:
STJ29289

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PTPRE (20-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPRE
Gene ID: 5791
Uniprot ID: PTPRE_HUMAN
Immunogen Region: 20-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human PTPRE (NP_006495.1).
Immunogen Sequence: LRGNETTADSNETTTTSGPP DPGASQPLLAWLLLPLLLLL LVLLLAAYFFRFRKQRKAVV STSDKKMPNGILEEQEQQRV MLLSRSPSGPKKYFPIPVEH LEEEIRIRSADDCKQFREEF NSLPSGHIQGTFELANKEEN REKNRYPNILPNDHSRVILS QLDGIPCSDYINASYIDGYK E
Tissue Specificity Expressed in giant cell tumor (osteoclastoma rich in multinucleated osteoclastic cells).
Post Translational Modifications A catalytically active cytoplasmic form (p65) is produced by proteolytic cleavage of either isoform 1, isoform 2 or isoform 3. Isoform 1 and isoform 2 are phosphorylated on tyrosine residues by tyrosine kinase Neu. Isoform 1 is glycosylated.
Function Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function. Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake. Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).
Protein Name Receptor-Type Tyrosine-Protein Phosphatase Epsilon
Protein-Tyrosine Phosphatase Epsilon
R-Ptp-Epsilon
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cytoplasm
Predominantly Cytoplasmic
A Small Fraction Is Also Associated With Nucleus And Membrane
Insulin Induces Translocation To The Membrane
Isoform 3: Cytoplasm
Alternative Antibody Names Anti-Receptor-Type Tyrosine-Protein Phosphatase Epsilon antibody
Anti-Protein-Tyrosine Phosphatase Epsilon antibody
Anti-R-Ptp-Epsilon antibody
Anti-PTPRE antibody

Information sourced from Uniprot.org

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