• Western blot analysis of extracts of various cell lines, using Parathyroid Hormone (Parathyroid Hormone (PTH)) antibody (STJ28409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 60s.
  • Immunofluorescence analysis of U2OS cells using Parathyroid Hormone (Parathyroid Hormone (PTH)) antibody (STJ28409). Blue: DAPI for nuclear staining.

Anti-PTH-antibody (aa20-115) (STJ28409)

SKU:
STJ28409

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Host: Rabbit
Applications: WB, IF
Reactivity: Human
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-PTH is suitable for use in Western Blot and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:10-1:100
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: PTH
Gene ID: 5741
Uniprot ID: PTHY_HUMAN
Immunogen Region: 20-115
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-115 of human PTH (NP_000306.1).
Function PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates 1-14C-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Protein Name Parathyroid Hormone
Pth
Parathormone
Parathyrin
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-418555
Reactome: R-HSA-9660821
Cellular Localisation Secreted
Alternative Antibody Names Anti-Parathyroid Hormone antibody
Anti-Pth antibody
Anti-Parathormone antibody
Anti-Parathyrin antibody
Anti-PTH antibody

Information sourced from Uniprot.org

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