• Western blot analysis of extracts of various cell lines, using PSMA8 antibody (STJ116577) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PSMA8 antibody (1-240) (STJ116577)

SKU:
STJ116577

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PSMA8 (1-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PSMA8
Gene ID: 143471
Uniprot ID: PSMA8_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PSMA8 (NP_653263.2).
Immunogen Sequence: MASRYDRAITVFSPDGHLFQ VEYAQEAVKKGSTAVGIRGT NIVVLGVEKKSVAKLQDERT VRKICALDDHVCMAFAVLTI FIGLTADARVVINRARVECQ SHKLTVEDPVTVEYITRFIA TLKQKYTQSNGRRPFGISAL IVGFDDDGISRLYQTDPSGT YHAWKANAIGRSAKTVREFL EKNYTEDAIASDSEAIKLAI KALLEVVQSGGKNIELAIIR RNQPLKMFSAKEVELYVTE
Function Component of the spermatoproteasome, a proteasome specifically found in testis that promotes acetylation-dependent degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. The proteasome is a protein complex that degrades unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. Required for 20S core proteasome assembly, essential for the degradation of meiotic proteins RAD51 and RPA1 at late prophase I and the progression of meiosis I during spermatogenesis. Localizes to the synaptonemal complex, a 'zipper'-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I.
Protein Name Proteasome Subunit Alpha-Type 8
Proteasome Alpha 4 Subunit
Alpha4s
Proteasome Subunit Alpha-Type 7-Like
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1234176
Reactome: R-HSA-1236974
Reactome: R-HSA-1236978
Reactome: R-HSA-174113
Reactome: R-HSA-174154
Reactome: R-HSA-174178
Reactome: R-HSA-174184
Reactome: R-HSA-180534
Reactome: R-HSA-180585
Reactome: R-HSA-195253
Reactome: R-HSA-202424
Reactome: R-HSA-211733
Reactome: R-HSA-2467813
Reactome: R-HSA-2871837
Reactome: R-HSA-349425
Reactome: R-HSA-350562
Reactome: R-HSA-382556
Reactome: R-HSA-450408
Reactome: R-HSA-4608870
Reactome: R-HSA-4641257
Reactome: R-HSA-4641258
Reactome: R-HSA-5358346
Reactome: R-HSA-5362768
Reactome: R-HSA-5607761
Reactome: R-HSA-5607764
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5632684
Reactome: R-HSA-5658442
Reactome: R-HSA-5668541
Reactome: R-HSA-5676590
Reactome: R-HSA-5678895
Reactome: R-HSA-5687128
Reactome: R-HSA-5689603
Reactome: R-HSA-5689880
Reactome: R-HSA-68949
Reactome: R-HSA-69017
Reactome: R-HSA-69481
Reactome: R-HSA-69601
Reactome: R-HSA-75815
Reactome: R-HSA-8852276
Reactome: R-HSA-8854050
Reactome: R-HSA-8939236
Reactome: R-HSA-8939902
Reactome: R-HSA-8948751
Reactome: R-HSA-8951664
Reactome: R-HSA-9010553
Reactome: R-HSA-9020702
Reactome: R-HSA-9755511
Reactome: R-HSA-983168
Cellular Localisation Nucleus
Localizes To The Central Region Of The Synaptonemal Complex
Alternative Antibody Names Anti-Proteasome Subunit Alpha-Type 8 antibody
Anti-Proteasome Alpha 4 Subunit antibody
Anti-Alpha4s antibody
Anti-Proteasome Subunit Alpha-Type 7-Like antibody
Anti-PSMA8 antibody
Anti-PSMA7L antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance