| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-PRKACB (1-351) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PRKACB |
| Gene ID: | 5567 |
| Uniprot ID: | KAPCB_HUMAN |
| Immunogen Region: | 1-351 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human PKA C-beta (PRKACB) (NP_002722.1). |
| Immunogen Sequence: | MGNAATAKKGSEVESVKEFL AKAKEDFLKKWENPTQNNAG LEDFERKKTLGTGSFGRVML VKHKATEQYYAMKILDKQKV VKLKQIEHTLNEKRILQAVN FPFLVRLEYAFKDNSNLYMV MEYVPGGEMFSHLRRIGRFS EPHARFYAAQIVLTFEYLHS LDLIYRDLKPENLLIDHQGY IQVTDFGFAKRVKGRTWTLC GTPEYLAPEIILSKGYNKAV DWWALGVLIYEMAAGYPPF |
| Tissue Specificity | Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. |
| Post Translational Modifications | Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. |
| Function | Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA. Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR. |
| Protein Name | Camp-Dependent Protein Kinase Catalytic Subunit BetaPka C-Beta |
| Database Links | Reactome: R-HSA-111931Reactome: R-HSA-163358Reactome: R-HSA-163560Reactome: R-HSA-163615Reactome: R-HSA-164378Reactome: R-HSA-180024Reactome: R-HSA-381676Reactome: R-HSA-392517Reactome: R-HSA-422356Reactome: R-HSA-432040Reactome: R-HSA-4420097Reactome: R-HSA-442720Reactome: R-HSA-5610780Reactome: R-HSA-5610783Reactome: R-HSA-5610785Reactome: R-HSA-5610787Reactome: R-HSA-5621575Reactome: R-HSA-5687128Reactome: R-HSA-8853659Reactome: R-HSA-8963896Reactome: R-HSA-9010642Reactome: R-HSA-9634597Reactome: R-HSA-9634600Reactome: R-HSA-9660821Reactome: R-HSA-9664323Reactome: R-HSA-983231 |
| Cellular Localisation | CytoplasmCell MembraneMembraneLipid-AnchorNucleusTranslocates Into The Nucleus (Monomeric Catalytic Subunit)The Inactive Holoenzyme Is Found In The Cytoplasm |
| Alternative Antibody Names | Anti-Camp-Dependent Protein Kinase Catalytic Subunit Beta antibodyAnti-Pka C-Beta antibodyAnti-PRKACB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
