| Host: |
Mouse |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Perforin-1 (1-100 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
ABT365 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1k |
| Formulation: |
Liquid in PBS pH7.2, 0.03% Proclin 300, with stabilizing protein. |
| Purification: |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range: |
IHC-P 1:200-400WB 500-1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRF1 |
| Gene ID: |
5551 |
| Uniprot ID: |
PERF_HUMAN |
| Immunogen Region: |
1-100 aa |
| Specificity: |
The antibody can specifically recognize human Perforin protein. In western blotting of Jurkat cell lysate, the antibody can label a 61 kDa band corresponding to Perforin. |
| Immunogen: |
Synthesized peptide derived from the human Perforin at the amino acid range 1-100 |
| Function | Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu. |
| Protein Name | Perforin-1P1CytolysinLymphocyte Pore-Forming ProteinPfp |
| Cellular Localisation | Cytolytic GranuleSecretedCell MembraneMulti-Pass Membrane ProteinEndosome LumenStored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target CellInserts Into The Cell Membrane Of Target Cells And Forms PoresMembrane Insertion And Pore Formation Requires A Major Conformation ChangeMay Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes |
| Alternative Antibody Names | Anti-Perforin-1 antibodyAnti-P1 antibodyAnti-Cytolysin antibodyAnti-Lymphocyte Pore-Forming Protein antibodyAnti-Pfp antibodyAnti-PRF1 antibodyAnti-PFP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
