• Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3 (PRDX3) ) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 (PRDX3) Rabbit polyclonal antibody (STJ11100034) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-PRDX3 antibody (1-256) (STJ11100034)

SKU:
STJ11100034

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRDX3 (1-256) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRDX3
Gene ID: 10935
Uniprot ID: PRDX3_HUMAN
Immunogen Region: 1-256
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human Peroxiredoxin 3 (PRDX3) (NP_006784.1).
Immunogen Sequence: MAAAVGRLLRASVARHVSAI PWGISATAALRPAACGRTSL TNLLCSGSSQAKLFSTSSSC HAPAVTQHAPYFKGTAVVNG EFKDLSLDDFKGKYLVLFFY PLDFTFVCPTEIVAFSDKAN EFHDVNCEVVAVSVDSHFSH LAWINTPRKNGGLGHMNIAL LSDLTKQISRDYGVLLEGSG LALRGLFIIDPNGVIKHLSV NDLPVGRSVEETLRLVKAFQ YVETHGEVCPANWTPDSPT
Post Translational Modifications Phosphorylated by LRRK2.phosphorylation reduces perodixase activity. The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond. S-palmitoylated.
Function Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Required for the maintenance of physical strength.
Protein Name Thioredoxin-Dependent Peroxide Reductase - Mitochondrial
Antioxidant Protein 1
Aop-1
Hbc189
Peroxiredoxin Iii
Prx-Iii
Peroxiredoxin-3
Protein Mer5 Homolog
Thioredoxin-Dependent Peroxiredoxin 3
Database Links Reactome: R-HSA-3299685
Cellular Localisation Mitochondrion
Cytoplasm
Early Endosome
Localizes To Early Endosomes In A Rps6kc1-Dependent Manner
Alternative Antibody Names Anti-Thioredoxin-Dependent Peroxide Reductase - Mitochondrial antibody
Anti-Antioxidant Protein 1 antibody
Anti-Aop-1 antibody
Anti-Hbc189 antibody
Anti-Peroxiredoxin Iii antibody
Anti-Prx-Iii antibody
Anti-Peroxiredoxin-3 antibody
Anti-Protein Mer5 Homolog antibody
Anti-Thioredoxin-Dependent Peroxiredoxin 3 antibody
Anti-PRDX3 antibody
Anti-AOP1 antibody

Information sourced from Uniprot.org

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