• Western blot analysis of lysates from mouse liver, using PPT2 Rabbit polyclonal antibody (STJ114141) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PPT2 antibody (28-302) (STJ114141)

SKU:
STJ114141

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPT2 (28-302) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPT2
Gene ID: 9374
Uniprot ID: PPT2_HUMAN
Immunogen Region: 28-302
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-302 of human PPT2 (NP_005146.4).
Immunogen Sequence: APAPHRASYKPVIVVHGLFD SSYSFRHLLEYINETHPGTV VTVLDLFDGRESLRPLWEQV QGFREAVVPIMAKAPQGVHL ICYSQGGLVCRALLSVMDDH NVDSFISLSSPQMGQYGDTD YLKWLFPTSMRSNLYRICYS PWGQEFSICNYWHDPHHDDL YLNASSFLALINGERDHPNA TVWRKNFLRVGHLVLIGGPD DGVITPWQSSFFGFYDANET VLEMEEQLVYLRDSFGLKT
Tissue Specificity Broadly expressed, with highest levels in skeletal muscle.
Function Removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA. Has the highest S-thioesterase activity for the acyl groups palmitic and myristic acid followed by other short- and long-chain acyl substrates. However, because of structural constraints, is unable to remove palmitate from peptides or proteins.
Protein Name Lysosomal Thioesterase Ppt2
Ppt-2
S-Thioesterase G14
Database Links Reactome: R-HSA-75105
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Lysosomal Thioesterase Ppt2 antibody
Anti-Ppt-2 antibody
Anti-S-Thioesterase G14 antibody
Anti-PPT2 antibody

Information sourced from Uniprot.org

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