• Immunofluorescence analysis of NIH/3T3 cells using PPP3R1 antibody (STJ25094) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PPP3R1 antibody (STJ25094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PPP3R1 antibody (11-170) (STJ25094)

SKU:
STJ25094

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP3R1
Gene ID: 5534
Uniprot ID: CANB1_HUMAN
Immunogen Region: 11-170
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 11-170 of human PPP3R1 (NP_000936.1).
Immunogen Sequence: MCSHFDADEIKRLGKRFKKL DLDNSGSLSVEEFMSLPELQ QNPLVQRVIDIFDTDGNGEV DFKEFIEGVSQFSVKGDKEQ KLRFAFRIYDMDKDGYISNG ELFQVLKMMVGNNLKDTQLQ QIVDKTIINADKDGDGRISF EEFCAVVGGLDIHKKMVVDV
Function Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Protein Name Calcineurin Subunit B Type 1
Protein Phosphatase 2b Regulatory Subunit 1
Protein Phosphatase 3 Regulatory Subunit B Alpha Isoform 1
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-180024
Reactome: R-HSA-2025928
Reactome: R-HSA-2871809
Reactome: R-HSA-4086398
Reactome: R-HSA-5607763
Cellular Localisation Cytoplasm
Cytosol
Cell Membrane
Sarcolemma
Lipid-Anchor
Translocates From The Cytosol To The Sarcolemma In A Cib1-Dependent Manner During Cardiomyocyte Hypertrophy
Alternative Antibody Names Anti-Calcineurin Subunit B Type 1 antibody
Anti-Protein Phosphatase 2b Regulatory Subunit 1 antibody
Anti-Protein Phosphatase 3 Regulatory Subunit B Alpha Isoform 1 antibody
Anti-PPP3R1 antibody
Anti-CNA2 antibody
Anti-CNB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance