| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PPP1R9B |
| Gene ID: | 84687 |
| Uniprot ID: | NEB2_HUMAN |
| Immunogen Region: | 250-350 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human PPP1R9B (NP_115984.3). |
| Immunogen Sequence: | VFQPPPPPPPAPSGDAPAEK ERCPAGQQPPQHRVAPARPP PKPREVRKIKPVEVEESGES EAESAPGEVIQAEVTVHAAL ENGSTVATAASPAPEEPKAQ A |
| Post Translational Modifications | Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1. |
| Function | Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1. Required for hepatocyte growth factor (HGF)-induced cell migration. |
| Protein Name | Neurabin-2 Neurabin-Ii Protein Phosphatase 1 Regulatory Subunit 9b Spinophilin |
| Cellular Localisation | Cytoplasm Cytoskeleton Nucleus Cell Projection Dendritic Spine Postsynaptic Density Synapse Cell Junction Adherens Junction Cell Membrane Lamellipodium Filopodium Ruffle Membrane Enriched At Synapse And Cadherin-Based Cell-Cell Adhesion Sites In Neurons Both Cytosolic And Membrane-Associated And Highly Enriched In The Postsynaptic Density Apposed To Exitatory Synapses Colocalizes With Ppp1r2 At Actin-Rich Adherens Junctions In Epithelial Cells And In Dendritic Spines Accumulates In The Lamellipodium Filopodium And Ruffle Membrane In Response To Hepatocyte Growth Factor (Hgf) Treatment |
| Alternative Antibody Names | Anti-Neurabin-2 antibody Anti-Neurabin-Ii antibody Anti-Protein Phosphatase 1 Regulatory Subunit 9b antibody Anti-Spinophilin antibody Anti-PPP1R9B antibody Anti-PPP1R6 antibody |
Information sourced from Uniprot.org