• Immunofluorescence analysis of U-2 OS cells using PP1 beta Rabbit monoclonal antibody (STJ11102056) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PP1 beta Rabbit monoclonal antibody (STJ11102056) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PP1 beta in paraffin-embedded human pancreas tissue using PP1 beta Rabbit monoclonal antibody (STJ11102056) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PP1 beta in paraffin-embedded human esophagus tissue using PP1 beta Rabbit monoclonal antibody (STJ11102056) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PP1 beta in paraffin-embedded human breast cancer tissue using PP1 beta Rabbit monoclonal antibody (STJ11102056) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PP1 beta in paraffin-embedded mouse colon tissue using PP1 beta Rabbit monoclonal antibody (STJ11102056) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PP1 beta in paraffin-embedded rat colon tissue using PP1 beta Rabbit monoclonal antibody (STJ11102056) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using PP1 beta Rabbit monoclonal antibody (STJ11102056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PPP1CB antibody (228-327) [S6MR] (STJ11102056)

SKU:
STJ11102056

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Protein Phosphatase 1 beta (228-327) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1CB
Gene ID: 5500
Uniprot ID: PP1B_HUMAN
Immunogen Region: 228-327
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 228-327 of human PP1 beta (P62140).
Immunogen Sequence: ADVVSKFLNRHDLDLICRAH QVVEDGYEFFAKRQLVTLFS APNYCGEFDNAGGMMSVDET LMCSFQILKPSEKKAKYQYG GLNSGRPVTPPRTANPPKKR
Function Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective.
Protein Name Serine/Threonine-Protein Phosphatase Pp1-Beta Catalytic Subunit
Pp-1b
Ppp1cd
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-2173788
Reactome: R-HSA-2565942
Reactome: R-HSA-400253
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5627123
Reactome: R-HSA-5673000
Reactome: R-HSA-9726840
Reactome: R-HSA-9726842
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleolus
Highly Mobile In Cells And Can Be Relocalized Through Interaction With Targeting Subunits
In The Presence Of Ppp1r8 Relocalizes From The Nucleus To Nuclear Speckles
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase Pp1-Beta Catalytic Subunit antibody
Anti-Pp-1b antibody
Anti-Ppp1cd antibody
Anti-PPP1CB antibody

Information sourced from Uniprot.org

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