• Western blot analysis of extracts of various cell lines, using PPIH antibody (STJ114163) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PPIH antibody (1-177) (STJ114163)

SKU:
STJ114163

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPIH (1-177) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPIH
Gene ID: 10465
Uniprot ID: PPIH_HUMAN
Immunogen Region: 1-177
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-177 of human PPIH (NP_006338.1).
Immunogen Sequence: MAVANSSPVNPVVFFDVSIG GQEVGRMKIELFADVVPKTA ENFRQFCTGEFRKDGVPIGY KGSTFHRVIKDFMIQGGDFV NGDGTGVASIYRGPFADENF KLRHSAPGLLSMANSGPSTN GCQFFITCSKCDWLDGKHVV FGKIIDGLLVMRKIENVPTG PNNKPKLPVVISQCGEM
Function PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase H
Ppiase H
Rotamase H
Small Nuclear Ribonucleoprotein Particle-Specific Cyclophilin H
Cyph
U-Snrnp-Associated Cyclophilin Snucyp-20
Usa-Cyp
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-9692916
Cellular Localisation Nucleus Speckle
Cytoplasm
Colocalizes With Spliceosomal Snrnps
A Small Proportion May Also Be Cytoplasmic
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase H antibody
Anti-Ppiase H antibody
Anti-Rotamase H antibody
Anti-Small Nuclear Ribonucleoprotein Particle-Specific Cyclophilin H antibody
Anti-Cyph antibody
Anti-U-Snrnp-Associated Cyclophilin Snucyp-20 antibody
Anti-Usa-Cyp antibody
Anti-PPIH antibody
Anti-CYP20 antibody
Anti-CYPH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance