| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Periphilin-1 (71-120 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PPHLN1 |
| Gene ID: | 51535 |
| Uniprot ID: | PPHLN_HUMAN |
| Immunogen Region: | 71-120 aa |
| Specificity: | Periphilin 1 Polyclonal Antibody detects endogenous levels of Periphilin 1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human PPHLN at the amino acid range 71-120 |
| Post Translational Modifications | Substrate of transglutaminase (in vitro). |
| Function | Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin. Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex. The HUSH complex is also involved in the silencing of unintegrated retroviral DNA: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed. May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation. |
| Protein Name | Periphilin-1 Cdc7 Expression Repressor Cr Gastric Cancer Antigen Ga50 |
| Database Links | Reactome: R-HSA-9843970 |
| Cellular Localisation | Nucleus Cytoplasm Chromosome In Undifferentiated Keratinocytes Expressed In Speckle-Type Nuclear Granules And At The Nuclear Membrane But In The Differentiated Keratinocytes Colocalized With Periplakin At The Cell Periphery And At Cell-Cell Junctions Localizes To Chromatin |
| Alternative Antibody Names | Anti-Periphilin-1 antibody Anti-Cdc7 Expression Repressor antibody Anti-Cr antibody Anti-Gastric Cancer Antigen Ga50 antibody Anti-PPHLN1 antibody Anti-HSPC206 antibody Anti-HSPC232 antibody |
Information sourced from Uniprot.org