• Immunofluorescence analysis of A-549 cells using POU6F1 antibody (STJ29438).
  • Western blot analysis of extracts of various cell lines, using POU6F1 antibody (STJ29438) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-POU6F1 antibody (1-301) (STJ29438)

SKU:
STJ29438

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POU6F1 (1-301) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU6F1
Gene ID: 5463
Uniprot ID: PO6F1_HUMAN
Immunogen Region: 1-301
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human POU6F1 (NP_002693.3).
Immunogen Sequence: MPGISSQILTNAQGQVIGTL PWVVNSASVAAPAPAQSLQV QAVTPQLLLNAQGQVIATLA SSPLPPPVAVRKPSTPESPA KSEVQPIQPTPTVPQPAVVI ASPAPAAKPSASAPIPITCS ETPTVSQLVSKPHTPSLDED GINLEEIREFAKNFKIRRLS LGLTQTQVGQALTATEGPAY SQSAICRFEKLDITPKSAQK LKPVLEKWLNEAELRNQEGQ QNLMEFVGGEPSKKRKRRT
Tissue Specificity In the embryo, expressed exclusively in the developing brain, whereas in the adult its expression is restricted to brain, heart, skeletal muscle and lung. In the brain, the highest expression levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.
Function Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3').
Protein Name Pou Domain - Class 6 - Transcription Factor 1
Brain-Specific Homeobox/Pou Domain Protein 5
Brain-5
Brn-5
Mpou Homeobox Protein
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 6 - Transcription Factor 1 antibody
Anti-Brain-Specific Homeobox/Pou Domain Protein 5 antibody
Anti-Brain-5 antibody
Anti-Brn-5 antibody
Anti-Mpou Homeobox Protein antibody
Anti-POU6F1 antibody
Anti-BRN5 antibody
Anti-MPOU antibody
Anti-TCFB1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance