• Immunohistochemistry analysis of paraffin-embedded mouse liver using POU2F1/POU2F1/OCT1 Rabbit monoclonal antibody (STJ11101924) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using POU2F1/POU2F1/OCT1 Rabbit monoclonal antibody (STJ11101924) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using POU2F1/POU2F1/OCT1 Rabbit monoclonal antibody (STJ11101924) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-POU2F1 antibody (1-162) [S4MR] (STJ11101924)

SKU:
STJ11101924

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-POU2F1 (1-162) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000 IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU2F1
Gene ID: 5451
Uniprot ID: PO2F1_HUMAN
Immunogen Region: 1-162
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human POU2F1/POU2F1/OCT1 (P14859).
Immunogen Sequence: MNNPSETSKPSMESGDGNTG TQTNGLDFQKQPVPVGGAIS TAQAQAFLGHLHQVQLAGTS LQAAAQSLNVQSKSNEESGD SQQPSQPSQQPSVQAAIPQT QLMLAGGQITGLTLTPAQQQ LLLQQAQAQAQLLAAAVQQH SASQQHSAAGATISASAATP MT
Tissue Specificity Ubiquitous. Isoform 2 is lymphocyte-specific.
Post Translational Modifications Phosphorylated by PRKDC.
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. (Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Protein Name Pou Domain - Class 2 - Transcription Factor 1
Nf-A1
Octamer-Binding Protein 1
Oct-1
Octamer-Binding Transcription Factor 1
Otf-1
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-6807505
Reactome: R-HSA-749476
Reactome: R-HSA-76071
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 2 - Transcription Factor 1 antibody
Anti-Nf-A1 antibody
Anti-Octamer-Binding Protein 1 antibody
Anti-Oct-1 antibody
Anti-Octamer-Binding Transcription Factor 1 antibody
Anti-Otf-1 antibody
Anti-POU2F1 antibody
Anti-OCT1 antibody
Anti-OTF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance