• Western blot analysis of extracts of SW620 cells, using POLR2F antibody (STJ25049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLR2F antibody (1-127) (STJ25049)

SKU:
STJ25049

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLR2F (1-127) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLR2F
Gene ID: 5435
Uniprot ID: RPAB2_HUMAN
Immunogen Region: 1-127
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human POLR2F (NP_068809.1).
Immunogen Sequence: MSDNEDNFDGDDFDDVEEDE GLDDLENAEEEGQENVEILP SGERPQANQKRITTPYMTKY ERARVLGTRALQIAMCAPVM VELEGETDPLLIAMKELKAR KIPIIIRRYLPDGSYEDWGV DELIITD
Function DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds.
Protein Name Dna-Directed Rna Polymerases I - Ii - And Iii Subunit Rpabc2
Rna Polymerases I - Ii - And Iii Subunit Abc2
Dna-Directed Rna Polymerase Ii Subunit F
Dna-Directed Rna Polymerases I - Ii - And Iii 14.4 Kda Polypeptide
Rpabc14.4
Rpb14.4
Rpb6 Homolog
Rpc15
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167242
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-168325
Reactome: R-HSA-1834949
Reactome: R-HSA-203927
Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-5578749
Reactome: R-HSA-5601884
Reactome: R-HSA-5617472
Reactome: R-HSA-674695
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6796648
Reactome: R-HSA-6803529
Reactome: R-HSA-6807505
Reactome: R-HSA-72086
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-72203
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-73780
Reactome: R-HSA-73863
Reactome: R-HSA-73980
Reactome: R-HSA-749476
Reactome: R-HSA-75953
Reactome: R-HSA-75955
Reactome: R-HSA-76042
Reactome: R-HSA-76061
Reactome: R-HSA-76066
Reactome: R-HSA-76071
Reactome: R-HSA-77075
Reactome: R-HSA-8851708
Reactome: R-HSA-9018519
Reactome: R-HSA-9670095
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna-Directed Rna Polymerases I - Ii - And Iii Subunit Rpabc2 antibody
Anti-Rna Polymerases I - Ii - And Iii Subunit Abc2 antibody
Anti-Dna-Directed Rna Polymerase Ii Subunit F antibody
Anti-Dna-Directed Rna Polymerases I - Ii - And Iii 14.4 Kda Polypeptide antibody
Anti-Rpabc14.4 antibody
Anti-Rpb14.4 antibody
Anti-Rpb6 Homolog antibody
Anti-Rpc15 antibody
Anti-POLR2F antibody
Anti-POLRF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance