• Western blot analysis of extracts of various cell lines, using POLK antibody (STJ27875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLK antibody (1-260) (STJ27875)

SKU:
STJ27875

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLK (1-260) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLK
Gene ID: 51426
Uniprot ID: POLK_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLK (NP_057302.1).
Immunogen Sequence: MDSTKEKCDSYKDDLLLRMG LNDNKAGMEGLDKEKINKII MEATKGSRFYGNELKKEKQV NQRIENMMQQKAQITSQQLR KAQLQVDRFAMELEQSRNLS NTIVHIDMDAFYAAVEMRDN PELKDKPIAVGSMSMLSTSN YHARRFGVRAAMPGFIAKRL CPQLIIVPPNFDKYRAVSKE VKEILADYDPNFMAMSLDEA YLNITKHLEERQNWPEDKRR YFIKMGSSVENDNPGKEVN
Tissue Specificity Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
Function DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Protein Name Dna Polymerase Kappa
Dinb Protein
Dinp
Database Links Reactome: R-HSA-5655862
Reactome: R-HSA-5656169
Reactome: R-HSA-5685942
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Cellular Localisation Nucleus
Detected Throughout The Nucleus And At Replication Foci
Recruited To Dna Damage Sites In Response To Ultraviolet Irradiation: N6-Methyladenosine (M6a)-Containing Mrnas Accumulate In The Vicinity Of Dna Damage Sites And Their Presence Is Required To Recruit Polk
Alternative Antibody Names Anti-Dna Polymerase Kappa antibody
Anti-Dinb Protein antibody
Anti-Dinp antibody
Anti-POLK antibody
Anti-DINB1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance