| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IHC-P:1:100-1:500IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POLD3 |
| Gene ID: |
10714 |
| Uniprot ID: |
DPOD3_HUMAN |
| Immunogen Region: |
197-466 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1). |
| Immunogen Sequence: |
ASKAAAKTQETNKETKTEAK EVTNASAAGNKAPGKGNMMS NFFGKAAMNKFKVNLDSEQA VKEEKIVEQPTVSVTEPKLA TPAGLKKSSKKAEPVKVLQK EKKRGKRVALSDDETKETEN MRKKRRRIKLPESDSSEDEV FPDSPGAYEAESPSPPPPPS PPLEPVPKTEPEPPSVKSSS GENKRKRKRVLKSKTYLDGE GCIVTEKVYESESCTDSEEE LNMKTSSVHRPPAMTVKKE |
| Post Translational Modifications | Ubiquitinated, but not targeted to the proteasome. Sumoylated. Sumoylation with SUMO3 may be predominant. Phosphorylation at Ser-458 is catalyzed in vitro by PKA. It is thought to decrease the affinity for PCNA and Pol-delta4 processivity. Can also be phosphorylated in vitro by CDK1-cyclin-A complex, as well as CDK2-cyclin-A and CDK2-cyclin-E complexes. PCNA interferes with CDK-cyclin phosphorylation. |
| Function | Accessory component of both the DNA polymerase delta complex and the DNA polymerase zeta complex. As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS, as a component of the tetrameric DNA polymerase zeta complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the DNA polymerase zeta complex compared to the minimal zeta complex, consisting of only REV3L and REV7. |
| Protein Name | Dna Polymerase Delta Subunit 3Dna Polymerase Delta Subunit CDna Polymerase Delta Subunit P66Dna Polymerase Delta Subunit P68 |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-174411Reactome: R-HSA-174414Reactome: R-HSA-174417Reactome: R-HSA-174437Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5651801Reactome: R-HSA-5656169Reactome: R-HSA-5685942Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183 |
| Cellular Localisation | CytoplasmNucleusPartially Colocalizes With Pcna And Pold1 At S Phase Replication SitesRecruited To Dna Damage Sites Within 2 Hours Following Uv Irradiation |
| Alternative Antibody Names | Anti-Dna Polymerase Delta Subunit 3 antibodyAnti-Dna Polymerase Delta Subunit C antibodyAnti-Dna Polymerase Delta Subunit P66 antibodyAnti-Dna Polymerase Delta Subunit P68 antibodyAnti-POLD3 antibodyAnti-KIAA0039 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
