| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PNPT1 (1-260) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PNPT1 |
| Gene ID: |
87178 |
| Uniprot ID: |
PNPT1_HUMAN |
| Immunogen Region: |
1-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PNPT1 (NP_149100.2). |
| Immunogen Sequence: |
MAACRYCCSCLRLRPLSDGP FLLPRRDRALTQLQVRALWS SAGSRAVAVDLGNRKLEISS GKLARFADGSAVVQSGDTAV MVTAVSKTKPSPSQFMPLVV DYRQKAAAAGRIPTNYLRRE IGTSDKEILTSRIIDRSIRP LFPAGYFYDTQVLCNLLAVD GVNEPDVLAINGASVALSLS DIPWNGPVGAVRIGIIDGEY VVNPTRKEMSSSTLNLVVAG APKSQIVMLEASAENILQQ |
| Function | RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration.regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity.degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells.specifically and selectively degrades miR-221, preferentially. Also plays a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA. |
| Protein Name | Polyribonucleotide Nucleotidyltransferase 1 - Mitochondrial3'-5' Rna Exonuclease Old35Pnpase Old-35Polynucleotide Phosphorylase 1Pnpase 1Polynucleotide Phosphorylase-Like Protein |
| Cellular Localisation | CytoplasmMitochondrion MatrixMitochondrion Intermembrane SpacePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Polyribonucleotide Nucleotidyltransferase 1 - Mitochondrial antibodyAnti-3'-5' Rna Exonuclease Old35 antibodyAnti-Pnpase Old-35 antibodyAnti-Polynucleotide Phosphorylase 1 antibodyAnti-Pnpase 1 antibodyAnti-Polynucleotide Phosphorylase-Like Protein antibodyAnti-PNPT1 antibodyAnti-PNPASE antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")