| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PLAG1 (33-288) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PLAG1 |
| Gene ID: |
5324 |
| Uniprot ID: |
PLAG1_HUMAN |
| Immunogen Region: |
33-288 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 33-288 of human PLAG1 (NP_002646.2). |
| Immunogen Sequence: |
NFPCQLCDKAFNSVEKLKVH SYSHTGERPYKCIQQDCTKA FVSKYKLQRHMATHSPEKTH KCNYCEKMFHRKDHLKNHLH THDPNKETFKCEECGKNYNT KLGFKRHLALHAATSGDLTC KVCLQTFESTGVLLEHLKSH AGKSSGGVKEKKHQCEHCDR RFYTRKDVRRHMVVHTGRKD FLCQYCAQRFGRKDHLTRHM KKSHNQELLKVKTEPVDFLD PFTCNVSVPIKDELLPVMS |
| Tissue Specificity | Expressed in fetal tissues such as lung, liver and kidney. Not detected or weak detection in normal adult tissues, but highly expressed in salivary gland with benign or malignant pleiomorphic adenomas with or without 8q12 aberrations, with preferential occurrence in benign tumors. |
| Post Translational Modifications | Sumoylated with SUMO1.which inhibits transcriptional activity, but does not affect nuclear localization. Blockers of sumoylation pathway such as SENP3 and inactive UBE2I increases transcriptional capacity. Sumoylation is increased in the presence of PIAS1. Acetylated by lysine acetyltransferase EP300.which activates transcriptional capacity. Lysine residues that are sumoylated also seem to be target for acetylation. |
| Function | Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia. |
| Protein Name | Zinc Finger Protein Plag1Pleiomorphic Adenoma Gene 1 Protein |
| Cellular Localisation | NucleusStrong Nucleolar Localization When Sumoylation Is Inhibited |
| Alternative Antibody Names | Anti-Zinc Finger Protein Plag1 antibodyAnti-Pleiomorphic Adenoma Gene 1 Protein antibodyAnti-PLAG1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
