• Western blot analysis of various lysates, using PLA2R1 antibody (STJ11105029) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PLA2R1 antibody [S5029RM] (STJ11105029)

SKU:
STJ11105029

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Secretory Phospholipase A2 Receptor is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5029RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLA2R1
Gene ID: 22925
Uniprot ID: PLA2R_HUMAN
Immunogen: Recombinant protein of human PLA2R1.
Tissue Specificity Expressed in podocytes (at protein level). Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level.
Post Translational Modifications The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form.
Function Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phospholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in pro-inflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis.
Protein Name Secretory Phospholipase A2 Receptor
Pla2-R
Pla2r
180 Kda Secretory Phospholipase A2 Receptor
C-Type Lectin Domain Family 13 Member C
M-Type Receptor Cleaved Into - Soluble Secretory Phospholipase A2 Receptor
Soluble Pla2-R
Soluble Pla2r
Database Links Reactome: R-HSA-1482788
Reactome: R-HSA-1482801
Reactome: R-HSA-1482839
Reactome: R-HSA-1482922
Reactome: R-HSA-1482925
Reactome: R-HSA-1483166
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Soluble Secretory Phospholipase A2 Receptor: Secreted
Isoform 2: Secreted
Alternative Antibody Names Anti-Secretory Phospholipase A2 Receptor antibody
Anti-Pla2-R antibody
Anti-Pla2r antibody
Anti-180 Kda Secretory Phospholipase A2 Receptor antibody
Anti-C-Type Lectin Domain Family 13 Member C antibody
Anti-M-Type Receptor Cleaved Into - Soluble Secretory Phospholipase A2 Receptor antibody
Anti-Soluble Pla2-R antibody
Anti-Soluble Pla2r antibody
Anti-PLA2R1 antibody
Anti-CLEC13C antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance