| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-85/88 kDa calcium-independent phospholipase A2 (Internal) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 ELISA 1:5000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PLA2G6 |
| Gene ID: | 8398 |
| Uniprot ID: | PLPL9_HUMAN |
| Immunogen Region: | Internal |
| Specificity: | Group VI iPLA2 Polyclonal Antibody detects endogenous levels of Group VI iPLA2 protein. |
| Immunogen: | Synthesized peptide derived from the Internal region of human Group VI iPLA2. |
| Function | Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles. Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position. Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content. Preferentially hydrolyzes oxidized polyunsaturated fatty acyl chains from cardiolipins, yielding monolysocardiolipins that can be reacylated with unoxidized fatty acyls to regenerate native cardiolipin species. Hydrolyzes oxidized glycerophosphoethanolamines present in pancreatic islets, releasing oxidized polyunsaturated fatty acids such as hydroxyeicosatetraenoates (HETEs). Has thioesterase activity toward fatty-acyl CoA releasing CoA-SH known to facilitate fatty acid transport and beta-oxidation in mitochondria particularly in skeletal muscle. Plays a role in regulation of membrane dynamics and homeostasis. Selectively hydrolyzes sn-2 arachidonoyl group in plasmalogen phospholipids, structural components of lipid rafts and myelin. Regulates F-actin polymerization at the pseudopods, which is required for both speed and directionality of MCP1/CCL2-induced monocyte chemotaxis. Targets membrane phospholipids to produce potent lipid signaling messengers. Generates lysophosphatidate (LPA, 1-acyl-glycerol-3-phosphate), which acts via G-protein receptors in various cell types. Has phospholipase A2 activity toward platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), likely playing a role in inactivation of this potent pro-inflammatory signaling lipid. In response to glucose, amplifies calcium influx in pancreatic beta cells to promote INS secretion. Isoform Ankyrin-iPLA2-1: Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms. Isoform Ankyrin-iPLA2-2: Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms. |
| Protein Name | 85/88 Kda Calcium-Independent Phospholipase A2 Cai-Pla2 2-Lysophosphatidylcholine Acylhydrolase Group Vi Phospholipase A2 Gvi Pla2 Intracellular Membrane-Associated Calcium-Independent Phospholipase A2 Beta Ipla2-Beta Palmitoyl-Coa Hydrolase Patatin-Like Phospholipase Domain-Containing Protein 9 Pnpla9 |
| Database Links | Reactome: R-HSA-1482788 Reactome: R-HSA-1482798 Reactome: R-HSA-1482839 Reactome: R-HSA-2029485 Reactome: R-HSA-6811436 |
| Cellular Localisation | Cytoplasm Cell Membrane Mitochondrion Cell Projection Pseudopodium Recruited To The Membrane-Enriched Pseudopods Upon Mcp1/Ccl2 Stimulation In Monocytes |
| Alternative Antibody Names | Anti-85/88 Kda Calcium-Independent Phospholipase A2 antibody Anti-Cai-Pla2 antibody Anti-2-Lysophosphatidylcholine Acylhydrolase antibody Anti-Group Vi Phospholipase A2 antibody Anti-Gvi Pla2 antibody Anti-Intracellular Membrane-Associated Calcium-Independent Phospholipase A2 Beta antibody Anti-Ipla2-Beta antibody Anti-Palmitoyl-Coa Hydrolase antibody Anti-Patatin-Like Phospholipase Domain-Containing Protein 9 antibody Anti-Pnpla9 antibody Anti-PLA2G6 antibody Anti-PLPLA9 antibody |
Information sourced from Uniprot.org