| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PLA2G6 (350-450) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Pla2g6 |
| Gene ID: |
53357 |
| Uniprot ID: |
PLPL9_MOUSE |
| Immunogen Region: |
350-450 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 350-450 of mouse PLA2G6 (NP_001185952.1). |
| Immunogen Sequence: |
GNTPLHLAMSKDNMEMVKAL IVFGAEVDTPNDFGETPALI ASKISKLITRKALLTLLKTV GADHHFPIIQGVSTEQGSAA ATHPLFSLDRTQPPAISLNN L |
| Tissue Specificity | Expressed in neurons of central and peripheral nervous system. Highly expressed in Purkinje cells in cerebellum and dorsal and ventral horn neurons in the spinal cord. Expressed in testis (at protein level). Expressed in skeletal muscle (at protein level). |
| Function | Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles. Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position. Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content. Preferentially hydrolyzes oxidized polyunsaturated fatty acyl chains from cardiolipins, yielding monolysocardiolipins that can be reacylated with unoxidized fatty acyls to regenerate native cardiolipin species. Hydrolyzes oxidized glycerophosphoethanolamines present in pancreatic islets, releasing oxidized polyunsaturated fatty acids such as hydroxyeicosatetraenoates (HETEs). Has thioesterase activity toward fatty-acyl CoA releasing CoA-SH known to facilitate fatty acid transport and beta-oxidation in mitochondria particularly in skeletal muscle. Plays a role in regulation of membrane dynamics and homeostasis. Selectively hydrolyzes sn-2 arachidonoyl group in plasmalogen phospholipids, structural components of lipid rafts and myelin. Regulates F-actin polymerization at the pseudopods, which is required for both speed and directionality of MCP1/CCL2-induced monocyte chemotaxis. Targets membrane phospholipids to produce potent lipid signaling messengers. Generates lysophosphatidate (LPA, 1-acyl-glycerol-3-phosphate), which acts via G-protein receptors in various cell types. Has phospholipase A2 activity toward platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), likely playing a role in inactivation of this potent pro-inflammatory signaling lipid. In response to glucose, amplifies calcium influx in pancreatic beta cells to promote INS secretion. |
| Protein Name | 85/88 Kda Calcium-Independent Phospholipase A2Cai-Pla22-Lysophosphatidylcholine AcylhydrolaseGroup Vi Phospholipase A2Gvi Pla2Intracellular Membrane-Associated Calcium-Independent Phospholipase A2 BetaIpla2-BetaPalmitoyl-Coa HydrolasePatatin-Like Phospholipase Domain-Containing Protein 9Pnpla9 |
| Database Links | Reactome: R-MMU-1482788Reactome: -MMU-1482839Reactome: -MMU-2029485Reactome: -MMU-6811436 |
| Cellular Localisation | CytoplasmCell MembraneMitochondrionCell ProjectionPseudopodiumRecruited To The Membrane-Enriched Pseudopods Upon Mcp1/Ccl2 Stimulation In Monocytes |
| Alternative Antibody Names | Anti-85/88 Kda Calcium-Independent Phospholipase A2 antibodyAnti-Cai-Pla2 antibodyAnti-2-Lysophosphatidylcholine Acylhydrolase antibodyAnti-Group Vi Phospholipase A2 antibodyAnti-Gvi Pla2 antibodyAnti-Intracellular Membrane-Associated Calcium-Independent Phospholipase A2 Beta antibodyAnti-Ipla2-Beta antibodyAnti-Palmitoyl-Coa Hydrolase antibodyAnti-Patatin-Like Phospholipase Domain-Containing Protein 9 antibodyAnti-Pnpla9 antibodyAnti-Pla2g6 antibodyAnti-Pnpla9 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
