• Confocal imaging of paraffin-embedded Mouse testis using PIWIL1 Rabbit monoclonal antibody (STJ11101883, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat testis using PIWIL1 Rabbit monoclonal antibody (STJ11101883, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of PIWIL1 in paraffin-embedded mouse kidney using PIWIL1 Rabbit monoclonal antibody (STJ11101883) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PIWIL1 Rabbit monoclonal antibody (STJ11101883) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PIWIL1 antibody (700-800) [S3MR] (STJ11101883)

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STJ11101883

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PIWIL1 (700-800) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIWIL1
Gene ID: 9271
Uniprot ID: PIWL1_HUMAN
Immunogen Region: 700-800
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PIWIL1 (Q96J94).
Immunogen Sequence: YRDGVGDGQLKTLVNYEVPQ FLDCLKSIGRGYNPRLTVIV VKKRVNTRFFAQSGGRLQNP LPGTVIDVEVTRPEWYDFFI VSQAVRSGSVSPTHYNVIYD N
Tissue Specificity Expressed in spermatocytes and spermatids. Also detected in prostate cancer (at protein level). Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells.detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3: Ubiquitously expressed, and specifically in CD34(+) hematopoietic progenitor cells but not in more differentiated cells.
Post Translational Modifications Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in late spermatids, leading to its degradation. Ubiquitination only takes place following piRNA-binding in adult testis. Ubiquitination and degradation in late spermatogenesis by APC/C is probably required to release RNF8 from the cytoplasm and promote histone to protamine exchange by RNF8.
Function Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis.RNF8 being released upon ubiquitination and degradation of PIWIL1. Isoform 3: May be a negative developmental regulator.
Protein Name Piwi-Like Protein 1
Database Links Reactome: R-HSA-5601884
Cellular Localisation Cytoplasm
Component Of The Meiotic Nuage
Also Named P Granule
A Germ-Cell-Specific Organelle Required To Repress Transposon Activity During Meiosis
Also Present In Chromatoid Body
Alternative Antibody Names Anti-Piwi-Like Protein 1 antibody
Anti-PIWIL1 antibody
Anti-HIWI antibody

Information sourced from Uniprot.org

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