• Western blot analysis of extracts of Rat liver, using PINK1 antibody (STJ113137) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using PINK1 antibody (STJ113137) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded human breast cancer using PINK1 rabbit polyclonal antibody (STJ113137) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using PINK1 rabbit polyclonal antibody (STJ113137) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of A549 cells using PINK1 rabbit polyclonal antibody (STJ113137) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PINK1 rabbit polyclonal antibody (STJ113137) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using PINK1 rabbit polyclonal antibody (STJ113137) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using PINK1 rabbit polyclonal antibody (STJ113137) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.

Anti-PINK1 antibody (143-581) (STJ113137)

SKU:
STJ113137

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PINK1 (143-581) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PINK1
Gene ID: 65018
Uniprot ID: PINK1_HUMAN
Immunogen Region: 143-581
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 143-581 of human PINK1 (NP_115785.1).
Immunogen Sequence: LDTRRLQGFRLEEYLIGQSI GKGCSAAVYEATMPTLPQNL EVTKSTGLLPGRGPGTSAPG EGQERAPGAPAFPLAIKMMW NISAGSSSEAILNTMSQELV PASRVALAGEYGAVTYRKSK RGPKQLAPHPNIIRVLRAFT SSVPLLPGALVDYPDVLPSR LHPEGLGHGRTLFLVMKNYP CTLRQYLCVNTPSPRLAAMM LLQLLEGVDHLVQQGIAHRD LKSDNILVELDPDGCPWLV
Tissue Specificity Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
Post Translational Modifications Proteolytically cleaved. In healthy cells, the precursor is continuously imported into the inner mitochondrial membrane (IMM), where it is proteolytically cleaved by mitochondrial-processing peptidase (MPP) and then undergoes further proteolytic cleavage by PARL or AFG3L2 to give rise to the 52 kDa short form. The 52 kDa short form is then released into the cytosol where it rapidly undergoes proteasome-dependent degradation. In unhealthy cells, when cellular stress conditions lead to the loss of mitochondrial membrane potential, mitochondrial import is impaired leading to the precursor accumulating on the outer mitochondrial membrane (OMM). If accumulation at the OMM fails and it is imported into the depolarized mitochondria, it undergoes cleavage by the IMM protease OMA1, promoting its subsequent degradation by the proteasome. Autophosphorylated. Loss of mitochondrial membrane potential results in the precursor accumulating on the outer mitochondrial membrane (OMM) where it is activated by autophosphorylation. Autophosphorylation at Ser-228 and Ser-402 is sufficient and essential for selective recruitment of PRKN to depolarized mitochondria, via PINK1-dependent phosphorylation of ubiquitin and maybe PRKN.
Function Serine/threonine-protein kinase which protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins such as PRKN and DNM1L, to coordinate mitochondrial quality control mechanisms that remove and replace dysfunctional mitochondrial components. Depending on the severity of mitochondrial damage and/or dysfunction, activity ranges from preventing apoptosis and stimulating mitochondrial biogenesis to regulating mitochondrial dynamics and eliminating severely damaged mitochondria via mitophagy. Mediates the translocation and activation of PRKN at the outer membrane (OMM) of dysfunctional/depolarized mitochondria. At the OMM of damaged mitochondria, phosphorylates pre-existing polyubiquitin chains at 'Ser-65', the PINK1-phosphorylated polyubiquitin then recruits PRKN from the cytosol to the OMM where PRKN is fully activated by phosphorylation at 'Ser-65' by PINK1. In damaged mitochondria, mediates the decision between mitophagy or preventing apoptosis by promoting PRKN-dependent poly- or monoubiquitination of VDAC1.polyubiquitination of VDAC1 by PRKN promotes mitophagy, while monoubiquitination of VDAC1 by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis. When cellular stress results in irreversible mitochondrial damage, functions with PRKN to promote clearance of damaged mitochondria via selective autophagy (mitophagy). The PINK1-PRKN pathway also promotes fission of damaged mitochondria by phosphorylating and thus promoting the PRKN-dependent degradation of mitochondrial proteins involved in fission such as MFN2. This prevents the refusion of unhealthy mitochondria with the mitochondrial network or initiates mitochondrial fragmentation facilitating their later engulfment by autophagosomes. Also promotes mitochondrial fission independently of PRKN and ATG7-mediated mitophagy, via the phosphorylation and activation of DNM1L. Regulates motility of damaged mitochondria by promoting the ubiquitination and subsequent degradation of MIRO1 and MIRO2.in motor neurons, this likely inhibits mitochondrial intracellular anterograde transport along the axons which probably increases the chance of the mitochondria undergoing mitophagy in the soma. Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10. Phosphorylates LETM1, positively regulating its mitochondrial calcium transport activity.
Protein Name Serine/Threonine-Protein Kinase Pink1 - Mitochondrial
Brpk
Pten-Induced Putative Kinase Protein 1
Database Links Reactome: R-HSA-5205685
Reactome: R-HSA-9614657
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Mitochondrion Inner Membrane
Cytoplasm
Cytosol
Localizes Mostly In Mitochondrion And The Two Smaller Proteolytic Processed Fragments Localize Mainly In Cytosol
When Mitochondria Lose Mitochondrial Membrane Potential Following Damage
Pink1 Import Is Arrested
Which Induces Its Accumulation In The Outer Mitochondrial Membrane
Where It Acquires Kinase Activity
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Pink1 - Mitochondrial antibody
Anti-Brpk antibody
Anti-Pten-Induced Putative Kinase Protein 1 antibody
Anti-PINK1 antibody

Information sourced from Uniprot.org

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