• Immunofluorescence analysis of NIH/3T3 cells using PI3 Kinase p85 alpha Rabbit polyclonal antibody (STJ113785) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using PI3 Kinase p85 alpha Rabbit polyclonal antibody (STJ113785) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PI3 Kinase p85 alpha Rabbit polyclonal antibody (STJ113785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PIK3R1 antibody (240-380) (STJ113785)

SKU:
STJ113785

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Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PI3 Kinase p85 alpha (240-380) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIK3R1
Gene ID: 5295
Uniprot ID: P85A_HUMAN
Immunogen Region: 240-380
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_852556.2).
Immunogen Sequence: EKFKREGNEKEIQRIMHNYD KLKSRISEIIDSRRRLEEDL KKQAAEYREIDKRMNSIKPD LIQLRKTRDQYLMWLTQKGV RQKKLNEWLGNENTEDQYSL VEDDEDLPHHDEKTWNVGSS NRNKAENLLRGKRDGTFLVR E
Tissue Specificity Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
Post Translational Modifications Polyubiquitinated in T-cells by CBLB.which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. In adipose tissue, polyubiquitinated by the BCR(KBTBD2) E3 ubiquitin ligase complex.recognized by KBTBD2 through the SH2 domains, undergoes 'Lys-48'-linked polyubiquitination leading to its degradation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608.phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.
Function Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
Protein Name Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha
Pi3-Kinase Regulatory Subunit Alpha
Pi3k Regulatory Subunit Alpha
Ptdins-3-Kinase Regulatory Subunit Alpha
Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha
Pi3-Kinase Subunit P85-Alpha
Ptdins-3-Kinase Regulatory Subunit P85-Alpha
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-112399
Reactome: R-HSA-114604
Reactome: R-HSA-1236382
Reactome: R-HSA-1250342
Reactome: R-HSA-1257604
Reactome: R-HSA-1266695
Reactome: R-HSA-1433557
Reactome: R-HSA-1660499
Reactome: R-HSA-180292
Reactome: R-HSA-1839117
Reactome: R-HSA-186763
Reactome: R-HSA-1963642
Reactome: R-HSA-198203
Reactome: R-HSA-201556
Reactome: R-HSA-202424
Reactome: R-HSA-2029485
Reactome: R-HSA-210993
Reactome: R-HSA-2219530
Reactome: R-HSA-2424491
Reactome: R-HSA-2730905
Reactome: R-HSA-373753
Reactome: R-HSA-388841
Reactome: R-HSA-389357
Reactome: R-HSA-416476
Reactome: R-HSA-430116
Reactome: R-HSA-4420097
Reactome: R-HSA-512988
Reactome: R-HSA-5637810
Reactome: R-HSA-5654689
Reactome: R-HSA-5654695
Reactome: R-HSA-5654710
Reactome: R-HSA-5654720
Reactome: R-HSA-5655253
Reactome: R-HSA-5655291
Reactome: R-HSA-5655302
Reactome: R-HSA-5655332
Reactome: R-HSA-5673001
Reactome: R-HSA-6785807
Reactome: R-HSA-6811558
Reactome: R-HSA-8851907
Reactome: R-HSA-8853659
Reactome: R-HSA-8980692
Reactome: R-HSA-9009391
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013420
Reactome: R-HSA-9013423
Reactome: R-HSA-9013424
Reactome: R-HSA-9027276
Reactome: R-HSA-9028335
Reactome: R-HSA-9035034
Reactome: R-HSA-912526
Reactome: R-HSA-912631
Reactome: R-HSA-9603381
Reactome: R-HSA-9607240
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Reactome: R-HSA-9670439
Reactome: R-HSA-9673767
Reactome: R-HSA-9673770
Reactome: R-HSA-9680350
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Reactome: R-HSA-9703465
Reactome: R-HSA-9703648
Reactome: R-HSA-9725370
Reactome: R-HSA-983695
Alternative Antibody Names Anti-Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha antibody
Anti-Pi3-Kinase Regulatory Subunit Alpha antibody
Anti-Pi3k Regulatory Subunit Alpha antibody
Anti-Ptdins-3-Kinase Regulatory Subunit Alpha antibody
Anti-Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha antibody
Anti-Pi3-Kinase Subunit P85-Alpha antibody
Anti-Ptdins-3-Kinase Regulatory Subunit P85-Alpha antibody
Anti-PIK3R1 antibody
Anti-GRB1 antibody

Information sourced from Uniprot.org

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