| Host: |
Goat |
| Applications: |
Pep-ELISA |
| Reactivity: |
Human/Mouse/Rat/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-PICT1/GLTSCR2 (Internal) is suitable for use in ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
ELISA-antibody detection limit dilution 1:32000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
NOP53 |
| Gene ID: |
29997 |
| Uniprot ID: |
NOP53_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_056525.2 |
| Specificity: |
This antibody is expected to recognise a sequence corresponding to aa 38-51 of human PICT1 protein. |
| Immunogen Sequence: |
CRRGPRNKKRGWRRL |
| Post Translational Modifications | Ubiquitin-mediated proteasomal degradation is regulated by c-JUN. It is associated with relocalization to the nucleoplasm and decreased homooligomerization. Phosphorylated upon DNA damage probably by ATM and DNA-PK.may regulate NOP53 degradation. |
| Function | Nucleolar protein which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis. In ribosome biogenesis, may also play a role in rRNA transcription. Also functions as a nucleolar sensor that regulates the activation of p53/TP53 in response to ribosome biogenesis perturbation, DNA damage and other stress conditions. DNA damage or perturbation of ribosome biogenesis disrupt the interaction between NOP53 and RPL11 allowing RPL11 transport to the nucleoplasm where it can inhibit MDM2 and allow p53/TP53 activation. It may also positively regulate the function of p53/TP53 in cell cycle arrest and apoptosis through direct interaction, preventing its MDM2-dependent ubiquitin-mediated proteasomal degradation. Originally identified as a tumor suppressor, it may also play a role in cell proliferation and apoptosis by positively regulating the stability of PTEN, thereby antagonizing the PI3K-AKT/PKB signaling pathway. May also inhibit cell proliferation and increase apoptosis through its interaction with NF2. May negatively regulate NPM1 by regulating its nucleoplasmic localization, oligomerization and ubiquitin-mediated proteasomal degradation. Thereby, may prevent NPM1 interaction with MYC and negatively regulate transcription mediated by the MYC-NPM1 complex. May also regulate cellular aerobic respiration. In the cellular response to viral infection, may play a role in the attenuation of interferon-beta through the inhibition of RIGI. |
| Protein Name | Ribosome Biogenesis Protein Nop53Glioma Tumor Suppressor Candidate Region Gene 2 ProteinProtein Interacting With Carboxyl Terminus 1Pict-1P60 |
| Cellular Localisation | NucleusNucleolusNucleoplasmIn The Nucleolus May Be More Specifically Localized To The Fibrillar CenterMainly Nucleolar It Relocalizes To The Nucleoplasm Under Specific Conditions Including Ribosomal Stress Enabling It To Interact And Regulate Nucleoplasmic Proteins Like P53/Tp53Also Detected In The Cytosol |
| Alternative Antibody Names | Anti-Ribosome Biogenesis Protein Nop53 antibodyAnti-Glioma Tumor Suppressor Candidate Region Gene 2 Protein antibodyAnti-Protein Interacting With Carboxyl Terminus 1 antibodyAnti-Pict-1 antibodyAnti-P60 antibodyAnti-NOP53 antibodyAnti-GLT antibodyAnti-GLTSCR2 antibodyAnti-PICT1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
