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Anti-PI 3-kinase p85 Alpha/Gamma antibody (436-485 aa) (STJ95078)

Western blot analysis of lysates from COS7 cells, treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma Antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using PI3-kinase p85-alpha/gamma Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PI3-kinase p85-alpha/gamma Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of various cells using PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody diluted at 1：1000
Western blot analysis of 293T COLO lysis using PI 3-kinase p85 Alpha/Gamma antibody.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)
Immunofluorescence analysis of rat-lung tissue. 1, PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of human-lung tissue. 1, PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PI 3-kinase p85 Alpha/Gamma antibody (436-485 aa) (STJ95078)

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STJ95078

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat/Monkey/Chicken
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phosphatidylinositol 3-kinase regulatory subunit alpha and Phosphatidylinositol 3-kinase regulatory subunit gamma (436-485 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA res

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:20000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PIK3R1 PIK3R3
Gene ID:	5295 8503
Uniprot ID:	P85A_HUMAN P55G_HUMAN
Immunogen Region:	436-485 aa
Specificity:	PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody detects endogenous levels of PI 3-kinase p85 Alpha/Gamma protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human PI3-kinase p85-alpha/gamma at the amino acid range 436-485

Post Translational Modifications	Polyubiquitinated in T-cells by CBLB.which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608.phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.
Function	Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
Protein Name	Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha Pi3-Kinase Regulatory Subunit Alpha Pi3k Regulatory Subunit Alpha Ptdins-3-Kinase Regulatory Subunit Alpha Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha Pi3-Kinase Subunit P85-Alpha Ptdins-3-Kinase Regulatory Subunit P85-Alpha
Database Links	Reactome: R-HSA-109704 Reactome: R-HSA-112399 Reactome: R-HSA-114604 Reactome: R-HSA-1236382 Reactome: R-HSA-1250342 Reactome: R-HSA-1257604 Reactome: R-HSA-1266695 Reactome: R-HSA-1433557 Reactome: R-HSA-1660499 Reactome: R-HSA-180292 Reactome: R-HSA-1839117 Reactome: R-HSA-186763 Reactome: R-HSA-1963642 Reactome: R-HSA-198203 Reactome: R-HSA-202424 Reactome: R-HSA-2029485 Reactome: R-HSA-210993 Reactome: R-HSA-2219530 Reactome: R-HSA-2424491 Reactome: R-HSA-2730905 Reactome: R-HSA-373753 Reactome: R-HSA-388841 Reactome: R-HSA-389357 Reactome: R-HSA-416476 Reactome: R-HSA-430116 Reactome: R-HSA-4420097 Reactome: R-HSA-512988 Reactome: R-HSA-5637810 Reactome: R-HSA-5654689 Reactome: R-HSA-5654695 Reactome: R-HSA-5654710 Reactome: R-HSA-5654720 Reactome: R-HSA-5655253 Reactome: R-HSA-5655291 Reactome: R-HSA-5655302 Reactome: R-HSA-5673001 Reactome: R-HSA-6785807 Reactome: R-HSA-6811558 Reactome: R-HSA-8851907 Reactome: R-HSA-8853334 Reactome: R-HSA-8853338 Reactome: R-HSA-8853659 Reactome: R-HSA-9009391 Reactome: R-HSA-9027276 Reactome: R-HSA-9028335 Reactome: R-HSA-912526 Reactome: R-HSA-912631 Reactome: R-HSA-9603381 Reactome: R-HSA-9607240 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9670439 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-983695
Alternative Antibody Names	Anti-Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha antibody Anti-Pi3-Kinase Regulatory Subunit Alpha antibody Anti-Pi3k Regulatory Subunit Alpha antibody Anti-Ptdins-3-Kinase Regulatory Subunit Alpha antibody Anti-Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha antibody Anti-Pi3-Kinase Subunit P85-Alpha antibody Anti-Ptdins-3-Kinase Regulatory Subunit P85-Alpha antibody Anti-PIK3R1 antibody Anti-GRB1 antibody

Information sourced from Uniprot.org

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