• Western blot analysis of extracts of various cell lines, using PHYH antibody (STJ28226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PHYH antibody (1-338) (STJ28226)

SKU:
STJ28226

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHYH (1-338) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHYH
Gene ID: 5264
Uniprot ID: PAHX_HUMAN
Immunogen Region: 1-338
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-338 of human PHYH (NP_006205.1).
Immunogen Sequence: MEQLRAAARLQIVLGHLGRP SAGAVVAHPTSGTISSASFH PQQFQYTLDNNVLTLEQRKF YEENGFLVIKNLVPDADIQR FRNEFEKICRKEVKPLGLTV MRDVTISKSEYAPSEKMITK VQDFQEDKELFRYCTLPEIL KYVECFTGPNIMAMHTMLIN KPPDSGKKTSRHPLHQDLHY FPFRPSDLIVCAWTAMEHIS RNNGCLVVLPGTHKGSLKPH DYPKWEGGVNKMFHGIQDY
Tissue Specificity Expressed in liver, kidney, and T-cells, but not in spleen, brain, heart, lung and skeletal muscle.
Function Catalyzes the 2-hydroxylation of not only racemic phytanoyl-CoA and the isomers of 3-methylhexadecanoyl-CoA, but also a variety of other mono-branched 3-methylacyl-CoA esters (with a chain length of at least seven carbon atoms) and straight-chain acyl-CoA esters (with a chain length longer than four carbon atoms). Does not hydroxylate long and very long straight chain acyl-CoAs or 2-methyl- and 4-methyl-branched acyl-CoAs.
Protein Name Phytanoyl-Coa Dioxygenase - Peroxisomal
Phytanic Acid Oxidase
Phytanoyl-Coa Alpha-Hydroxylase
Phyh
Database Links Reactome: R-HSA-389599
Reactome: R-HSA-9033241
Reactome: R-HSA-9033500
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-Phytanoyl-Coa Dioxygenase - Peroxisomal antibody
Anti-Phytanic Acid Oxidase antibody
Anti-Phytanoyl-Coa Alpha-Hydroxylase antibody
Anti-Phyh antibody
Anti-PHYH antibody
Anti-PAHX antibody

Information sourced from Uniprot.org

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