• Western blot analysis of extracts of Jurkat cells, using Phospho-SRC Family-Y416 antibody (STJ110839) at 1:1000 dilution. Jurkat cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time:.
  • Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g Phospho-SRC Family-Y416 polyclonal antibody (STJ110839). Western blot was performed from the immunoprecipitate using Phospho-Lck-Y416/FYN-Y416 polyclonal antibody (STJ110839) at a dilution of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes after serum-starvation overnight.
  • Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-SRC Family-Y416 polyclonal antibody (STJ110839). Western blot was performed from the immunoprecipitate using Phospho-Lck-Y416/FYN-Y416 polyclonal antibody (STJ110839) at a dilition of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes after serum-starvation overnight.
  • Western blot analysis of lysates from Jurkat cells, using Phospho-SRC Family-Y416 Rabbit polyclonal antibody (STJ110839) at 1:1000 dilution. Jurkat cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time:.

Anti-Phospho-Src Family-Y416 antibody (STJ110839)

SKU:
STJ110839

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Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-SRC Family-Y416 is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SRC
LCK
Gene ID: 6714
3932
2534
SRC_HUMAN
LCK_HUMAN
FYN_HUMAN
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Immunogen: A synthetic phosphorylated peptide around Y416 of human SRC FamilySRC (NP_005408.1).
Immunogen Sequence: NEYTA
Tissue Specificity P12931 - Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. Isoform 1: Expressed in spleen and liver. Isoform 2: Expressed in brain. Isoform 3: Expressed in brain.
P06239 - Expressed specifically in lymphoid cells.
P06241 - Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
P07948 - Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
P08631 - Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.
P07947 - Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis.
P12931 - Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. Isoform 1: Expressed in spleen and liver. Isoform 2: Expressed in brain. Isoform 3: Expressed in brain.
P06239 - Expressed specifically in lymphoid cells.
P06241 - Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
P07948 - Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
P08631 - Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.
P07947 - Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis.
P12931 - Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. Isoform 1: Expressed in spleen and liver. Isoform 2: Expressed in brain. Isoform 3: Expressed in brain.
P06239 - Expressed specifically in lymphoid cells.
P06241 - Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
P07948 - Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
P08631 - Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.
P07947 - Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis.
P12931 - Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. Isoform 1: Expressed in spleen and liver. Isoform 2: Expressed in brain. Isoform 3: Expressed in brain.
P06239 - Expressed specifically in lymphoid cells.
P06241 - Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
P07948 - Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
P08631 - Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.
P07947 - Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis.
P12931 - Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. Isoform 1: Expressed in spleen and liver. Isoform 2: Expressed in brain. Isoform 3: Expressed in brain.
P06239 - Expressed specifically in lymphoid cells.
P06241 - Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
P07948 - Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
P08631 - Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.
P07947 - Expressed in the epithelial cells of renal proximal tubules and stomach as well as hematopoietic cells in the bone marrow and spleen in the fetal tissues. In adult, expressed in epithelial cells of the renal proximal tubules and present in keratinocytes in the basal epidermal layer of epidermis.
Alternative Antibody Names Anti-Proto-Oncogene Tyrosine-Protein Kinase Src antibody
Anti-Proto-Oncogene C-Src antibody
Anti-Pp60c-Src antibody
Anti-P60-Src antibody
Anti-SRC antibody
Anti-SRC1 antibody
Anti-Tyrosine-Protein Kinase Lck antibody
Anti-Leukocyte C-Terminal Src Kinase antibody
Anti-Lsk antibody
Anti-Lymphocyte Cell-Specific Protein-Tyrosine Kinase antibody
Anti-Protein Yt16 antibody
Anti-Proto-Oncogene Lck antibody
Anti-T Cell-Specific Protein-Tyrosine Kinase antibody
Anti-P56-Lck antibody
Anti-LCK antibody
Anti-Tyrosine-Protein Kinase Fyn antibody
Anti-Proto-Oncogene Syn antibody
Anti-Proto-Oncogene C-Fyn antibody
Anti-Src-Like Kinase antibody
Anti-Slk antibody
Anti-P59-Fyn antibody
Anti-FYN antibody
Anti-Tyrosine-Protein Kinase Lyn antibody
Anti-Lck/Yes-Related Novel Protein Tyrosine Kinase antibody
Anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog antibody
Anti-P53lyn antibody
Anti-P56lyn antibody
Anti-LYN antibody
Anti-JTK8 antibody
Anti-Tyrosine-Protein Kinase Hck antibody
Anti-Hematopoietic Cell Kinase antibody
Anti-Hemopoietic Cell Kinase antibody
Anti-P59-Hck/P60-Hck antibody
Anti-P59hck antibody
Anti-P61hck antibody
Anti-HCK antibody
Anti-Tyrosine-Protein Kinase Yes antibody
Anti-Proto-Oncogene C-Yes antibody
Anti-P61-Yes antibody
Anti-YES1 antibody
Anti-YES antibody
Anti-Proto-Oncogene Tyrosine-Protein Kinase Src antibody
Anti-Proto-Oncogene C-Src antibody
Anti-Pp60c-Src antibody
Anti-P60-Src antibody
Anti-SRC antibody
Anti-SRC1 antibody
Anti-Tyrosine-Protein Kinase Lck antibody
Anti-Leukocyte C-Terminal Src Kinase antibody
Anti-Lsk antibody
Anti-Lymphocyte Cell-Specific Protein-Tyrosine Kinase antibody
Anti-Protein Yt16 antibody
Anti-Proto-Oncogene Lck antibody
Anti-T Cell-Specific Protein-Tyrosine Kinase antibody
Anti-P56-Lck antibody

Information sourced from Uniprot.org

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