| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S9MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMC1A |
| Gene ID: | 8243 |
| Uniprot ID: | SMC1A_HUMAN |
| Specificity: | A synthetic phosphorylated peptide around S957 of human SMC1 (Q14683). |
| Immunogen Sequence: | GSSQG |
| Post Translational Modifications | Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation. Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation. |
| Function | Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. |
| Protein Name | Structural Maintenance Of Chromosomes Protein 1a Smc Protein 1a Smc-1-Alpha Smc-1a Sb1.8 |
| Database Links | Reactome: R-HSA-1221632 Reactome: R-HSA-2467813 Reactome: R-HSA-2468052 Reactome: R-HSA-2470946 Reactome: R-HSA-2500257 Reactome: R-HSA-3108214 Reactome: R-HSA-9018519 |
| Cellular Localisation | Nucleus Chromosome Centromere Kinetochore Associates With Chromatin Before Prophase It Is Scattered Along Chromosome Arms During Prophase Most Of Cohesin Complexes Dissociate From Chromatin Probably Because Of Phosphorylation By Plk Except At Centromeres Where Cohesin Complexes Remain At Anaphase The Rad21 Subunit Of The Cohesin Complex Is Cleaved Leading To The Dissociation Of The Complex From Chromosomes Allowing Chromosome Separation In Germ Cells Cohesin Complex Dissociates From Chromatin At Prophase I And May Be Replaced By A Meiosis-Specific Cohesin Complex The Phosphorylated Form On Ser-957 And Ser-966 Associates With Chromatin During G1/S/G2 Phases But Not During M Phase Suggesting That Phosphorylation Does Not Regulate Cohesin Function Integral Component Of The Functional Centromere-Kinetochore Complex At The Kinetochore Region During Mitosis |
| Alternative Antibody Names | Anti-Structural Maintenance Of Chromosomes Protein 1a antibody Anti-Smc Protein 1a antibody Anti-Smc-1-Alpha antibody Anti-Smc-1a antibody Anti-Sb1.8 antibody Anti-SMC1A antibody Anti-DXS423E antibody Anti-KIAA0178 antibody Anti-SB1.8 antibody Anti-SMC1 antibody Anti-SMC1L1 antibody |
Information sourced from Uniprot.org