• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-LDHA-Y10 antibody (STJ11101026) at 1:1000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-LDHA-Y10 antibody (STJ11101026)

SKU:
STJ11101026

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-LDHA-Y10 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDHA
Gene ID: 3939
Uniprot ID: LDHA_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y10 of human LDHA (NP_001128711.1).
Immunogen Sequence: LIYNL
Tissue Specificity Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.
Post Translational Modifications ISGylated.
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Protein Name L-Lactate Dehydrogenase A Chain
Ldh-A
Cell Proliferation-Inducing Gene 19 Protein
Ldh Muscle Subunit
Ldh-M
Renal Carcinoma Antigen Ny-Ren-59
Database Links Reactome: R-HSA-70268
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-L-Lactate Dehydrogenase A Chain antibody
Anti-Ldh-A antibody
Anti-Cell Proliferation-Inducing Gene 19 Protein antibody
Anti-Ldh Muscle Subunit antibody
Anti-Ldh-M antibody
Anti-Renal Carcinoma Antigen Ny-Ren-59 antibody
Anti-LDHA antibody
Anti-PIG19 antibody

Information sourced from Uniprot.org

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