• Immunofluorescence analysis of HUVEC cells, using Kir6.2 (Phospho-Thr224) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HeLa cells, using Kir6.2 (Phospho-Thr224) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-KCNJ11-Thr224 antibody (190-239 aa) (STJ90722)

SKU:
STJ90722

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-ATP-sensitive inward rectifier potassium channel 11 channel Kir6.2-Thr224 (190-239 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KCNJ11
Gene ID: 3767
Uniprot ID: KCJ11_HUMAN
Immunogen Region: 190-239 aa
Specificity: Phospho-KIR6.2 (T224) Polyclonal Antibody detects endogenous levels of KIR6.2 protein only when phosphorylated at T224.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Kir6.2 around the phosphorylation site of Thr224 at the amino acid range 190-239
Function This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium.as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.
Protein Name Atp-Sensitive Inward Rectifier Potassium Channel 11
Ikatp
Inward Rectifier K(+ Channel Kir6.2
Potassium Channel - Inwardly Rectifying Subfamily J Member 11
Database Links Reactome: R-HSA-1296025
Reactome: R-HSA-382556
Reactome: R-HSA-422356
Reactome: R-HSA-5578775
Reactome: R-HSA-5678420
Reactome: R-HSA-5683177
Cellular Localisation Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp-Sensitive Inward Rectifier Potassium Channel 11 antibody
Anti-Ikatp antibody
Anti-Inward Rectifier K(+ Channel Kir6.2 antibody
Anti-Potassium Channel - Inwardly Rectifying Subfamily J Member 11 antibody
Anti-KCNJ11 antibody

Information sourced from Uniprot.org

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