• Western blot analysis of extracts of 293T cells, using Phospho-Jak3-Y980/981 antibody (STJ110868). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of extracts of 293T cells, using Phospho-Jak3-Y980/981 antibody (STJ110868). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.

Anti-Phospho-JAK3-Y980/981 antibody (STJ110868)

SKU:
STJ110868

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Jak3-Y980/981 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JAK3
Gene ID: 3718
Uniprot ID: JAK3_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y980 & Y981 of human Jak3 (NP_000206.2).
Immunogen Sequence: KDYYVV
Tissue Specificity In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.
Post Translational Modifications Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable).
Function Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A and STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
Protein Name Tyrosine-Protein Kinase Jak3
Janus Kinase 3
Jak-3
Leukocyte Janus Kinase
L-Jak
Database Links Reactome: R-HSA-1266695
Reactome: R-HSA-201556
Reactome: R-HSA-5673001
Reactome: R-HSA-6785807
Reactome: R-HSA-8854691
Reactome: R-HSA-8983432
Reactome: R-HSA-8985947
Reactome: R-HSA-9020558
Reactome: R-HSA-9020958
Reactome: R-HSA-912526
Reactome: R-HSA-9679191
Cellular Localisation Endomembrane System
Peripheral Membrane Protein
Cytoplasm
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Jak3 antibody
Anti-Janus Kinase 3 antibody
Anti-Jak-3 antibody
Anti-Leukocyte Janus Kinase antibody
Anti-L-Jak antibody
Anti-JAK3 antibody

Information sourced from Uniprot.org

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