Western blot analysis of various lysates using Phospho-Histone H1. 3-T17/Histone H1. 4-T17 Rabbit mAb (STJ11102629) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H1.3/Histone H1.4-T17/T17 antibody [S9MR] (STJ11102629)

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STJ11102629
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H1-3
H1-4
Gene ID: 3007
3008
Uniprot ID: H13_HUMAN
H14_HUMAN
Specificity: A synthetic phosphorylated peptide around T17 of human Histone H1.3H13 (NP_005311.1).
Immunogen Sequence: EKTPV
Tissue Specificity
Alternative Antibody Names Anti-Histone H1.3 antibody
Anti-Histone H1c antibody
Anti-Histone H1s-2 antibody
Anti-H1-3 antibody
Anti-H1F3 antibody
Anti-HIST1H1D antibody
Anti-Histone H1.4 antibody
Anti-Histone H1b antibody
Anti-Histone H1s-4 antibody
Anti-H1-4 antibody
Anti-H1F4 antibody
Anti-HIST1H1E antibody

Information sourced from Uniprot.org

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