• Western blot analysis of extracts of RAW264. 7 cells, using Phospho-GATA3-S308 rabbit monoclonal antibody (STJ11102600) at 1:1000 dilution. RAW264. 7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Phospho-GATA3-S308 antibody [S0MR] (STJ11102600)

SKU:
STJ11102600

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-GATA3-S308 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATA3
Gene ID: 2625
Uniprot ID: GATA3_HUMAN
Immunogen: A synthetic phosphorylated peptide around S308 of human GATA3 (P23771).
Immunogen Sequence: RLSAA
Tissue Specificity T-cells and endothelial cells.
Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression. Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.
Protein Name Trans-Acting T-Cell-Specific Transcription Factor Gata-3
Gata-Binding Factor 3
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-6785807
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Trans-Acting T-Cell-Specific Transcription Factor Gata-3 antibody
Anti-Gata-Binding Factor 3 antibody
Anti-GATA3 antibody

Information sourced from Uniprot.org

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