| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Protein FosB-Ser27 (12-61 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FOSB |
| Gene ID: |
2354 |
| Uniprot ID: |
FOSB_HUMAN |
| Immunogen Region: |
12-61 aa |
| Specificity: |
Phospho-Fos B (S27) Polyclonal Antibody detects endogenous levels of Fos B protein only when phosphorylated at S27. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human FosB around the phosphorylation site of Ser27 at the amino acid range 12-61 |
| Post Translational Modifications | Phosphorylated. Isoform 11: Phosphorylated at Ser-27 by CSNK2A1.phosphorylation increases protein stability and transactivation potential. |
| Function | Heterodimerizes with proteins of the JUN family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to gene promoters containing an AP-1 consensus sequence 5'-TGAGCTCA-3' and enhancing their transcriptional activity. As part of the AP-1 complex, facilitates enhancer selection together with cell-type-specific transcription factors by collaboratively binding to nucleosomal enhancers and recruiting the SWI/SNF (BAF) chromatin remodeling complex to establish accessible chromatin. Together with JUN, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway. Exhibits transactivation activity in vitro. Involved in the display of nurturing behavior towards newborns. May play a role in neurogenesis in the hippocampus and in learning and memory-related tasks by regulating the expression of various genes involved in neurogenesis, depression and epilepsy. Implicated in behavioral responses related to morphine reward and spatial memory. Isoform 11: Exhibits lower transactivation activity than isoform 1 in vitro. The heterodimer with JUN does not display any transcriptional activity, and may thereby act as an transcriptional inhibitor. May be involved in the regulation of neurogenesis in the hippocampus. May play a role in synaptic modifications in nucleus accumbens medium spiny neurons and thereby play a role in adaptive and pathological reward-dependent learning, including maladaptive responses involved in drug addiction. Seems to be more stably expressed with a half-life of ~9.5 hours in cell culture as compared to 1.5 hours half-life of isoform 1. |
| Protein Name | Protein FosbFosb Proto-Oncogene - Ap-1 Transcription Factor SubunitG0/G1 Switch Regulatory Protein 3Transcription Factor Ap-1 Subunit Fosb |
| Database Links | Reactome: R-HSA-9018519Reactome: R-HSA-9031628 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Protein Fosb antibodyAnti-Fosb Proto-Oncogene - Ap-1 Transcription Factor Subunit antibodyAnti-G0/G1 Switch Regulatory Protein 3 antibodyAnti-Transcription Factor Ap-1 Subunit Fosb antibodyAnti-FOSB antibodyAnti-G0S3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
