Anti-Phospho-Crk p38-Y221 antibody [ARC1640] (STJ11102643)


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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Rabbit monoclonal anti-Phospho-Crk p38-Y221 antibody is suitable for use in Western Blot.
Clonality: Monoclonal
Clone ID: ARC1640
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Gene ID: 1398
Gene Symbol: CRK
Immunogen: A phospho specific peptide corresponding to residues surrounding Y221 of human Crk p38
Post Translational Modifications Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. Isoform Crk-II: Phosphorylated by KIT. Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form.
Function Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.
Protein Name Adapter Molecule Crk
Proto-Oncogene C-Crk
Database Links Reactome: R-HSA-170984
Reactome: R-HSA-186763
Reactome: R-HSA-2029482
Reactome: R-HSA-372708
Reactome: R-HSA-4420097
Reactome: R-HSA-8849471
Reactome: R-HSA-8875555
Reactome: R-HSA-8875656
Reactome: R-HSA-912631
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cell Membrane
Translocated To The Plasma Membrane Upon Cell Adhesion
Alternative Antibody Names Anti-Adapter Molecule Crk antibody
Anti-Proto-Oncogene C-Crk antibody
Anti-P38 antibody
Anti-CRK antibody

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