| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-IKK Alpha-S176 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CHUK |
| Gene ID: |
1147 |
| Uniprot ID: |
IKKA_HUMAN |
| Immunogen: |
A synthetic phosphorylated peptide around S176 of human IKK Alpha (NP_001269.3). |
| Immunogen Sequence: |
QGSLC |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated. Ubiquitinated by TRIM56 via 'Lys-63'-linked ubiquitination, promoting activation of CHUK/IKKA. (Microbial infection) Acetylation of Thr-179 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway. |
| Function | Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates AMBRA1 following mitophagy induction, promoting AMBRA1 interaction with ATG8 family proteins and its mitophagic activity. |
| Protein Name | Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit AlphaI-Kappa-B Kinase AlphaIkk-AIkk-AlphaIkbkaIkappab KinaseConserved Helix-Loop-Helix Ubiquitous KinaseI-Kappa-B Kinase 1Ikk-1Ikk1Nuclear Factor Nf-Kappa-B Inhibitor Kinase AlphaNfkbikaTranscription Factor 16Tcf-16 |
| Database Links | Reactome: R-HSA-1169091Reactome: R-HSA-1236974Reactome: R-HSA-168638Reactome: R-HSA-168927Reactome: R-HSA-1810476Reactome: R-HSA-198323Reactome: R-HSA-202424Reactome: R-HSA-2871837Reactome: R-HSA-445989Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5602636Reactome: R-HSA-5603027Reactome: R-HSA-5603029Reactome: R-HSA-5607761Reactome: R-HSA-5607764Reactome: R-HSA-5674400Reactome: R-HSA-5676590Reactome: R-HSA-5684264Reactome: R-HSA-9020702Reactome: R-HSA-933542Reactome: R-HSA-933543Reactome: R-HSA-937039Reactome: R-HSA-937041Reactome: R-HSA-9705671Reactome: R-HSA-975144Reactome: R-HSA-9758274 |
| Cellular Localisation | CytoplasmNucleusShuttles Between The Cytoplasm And The Nucleus |
| Alternative Antibody Names | Anti-Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Alpha antibodyAnti-I-Kappa-B Kinase Alpha antibodyAnti-Ikk-A antibodyAnti-Ikk-Alpha antibodyAnti-Ikbka antibodyAnti-Ikappab Kinase antibodyAnti-Conserved Helix-Loop-Helix Ubiquitous Kinase antibodyAnti-I-Kappa-B Kinase 1 antibodyAnti-Ikk-1 antibodyAnti-Ikk1 antibodyAnti-Nuclear Factor Nf-Kappa-B Inhibitor Kinase Alpha antibodyAnti-Nfkbika antibodyAnti-Transcription Factor 16 antibodyAnti-Tcf-16 antibodyAnti-CHUK antibodyAnti-IKKA antibodyAnti-TCF16 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
