• Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using Calnexin (Phospho-Ser583) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Calnexin (Phospho-Ser583) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Calnexin (S583) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CANX-Ser583 antibody (543-592 aa) (STJ90201)

SKU:
STJ90201

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Calnexin-Ser583 (543-592 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CANX
Gene ID: 821
Uniprot ID: CALX_HUMAN
Immunogen Region: 543-592 aa
Specificity: Phospho-Calnexin (S583) Polyclonal Antibody detects endogenous levels of Calnexin protein only when phosphorylated at S583.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Calnexin around the phosphorylation site of Ser583 at the amino acid range 543-592
Function Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Protein Name Calnexin
Ip90
Major Histocompatibility Complex Class I Antigen-Binding Protein P88
P90
Database Links Reactome: R-HSA-168316
Reactome: R-HSA-2132295
Reactome: R-HSA-8984722
Reactome: R-HSA-901042
Reactome: R-HSA-9020956
Reactome: R-HSA-9683686
Reactome: R-HSA-9694548
Reactome: R-HSA-983170
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
The Palmitoylated Form Preferentially Localizes To The Perinuclear Rough Er
Alternative Antibody Names Anti-Calnexin antibody
Anti-Ip90 antibody
Anti-Major Histocompatibility Complex Class I Antigen-Binding Protein P88 antibody
Anti-P90 antibody
Anti-CANX antibody

Information sourced from Uniprot.org

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