• Immunohistochemistry analysis of paraffin-embedded mouse brain using PDP1 antibody (STJ28254) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using PDP1 antibody (STJ28254) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using PDP1 antibody (STJ28254) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PDP1 antibody (STJ28254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PDP1 antibody (278-537) (STJ28254)

SKU:
STJ28254

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDP1 (278-537) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDP1
Gene ID: 54704
Uniprot ID: PDP1_HUMAN
Immunogen Region: 278-537
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 278-537 of human PDP1 (NP_060914.2).
Immunogen Sequence: HVDGVDLHVANTGDSRAMLG VQEEDGSWSAVTLSNDHNAQ NERELERLKLEHPKSEAKSV VKQDRLLGLLMPFRAFGDVK FKWSIDLQKRVIESGPDQLN DNEYTKFIPPNYHTPPYLTA EPEVTYHRLRPQDKFLVLAT DGLWETMHRQDVVRIVGEYL TGMHHQQPIAVGGYKVTLGQ MHGLLTERRTKMSSVFEDQN AATHLIRHAVGNNEFGTVDH ERLSKMLSLPEELARMYRD
Function Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
Protein Name Pyruvate Dehydrogenase Acetyl-Transferring-Phosphatase 1 - Mitochondrial
Pdp 1
Protein Phosphatase 2c
Pyruvate Dehydrogenase Phosphatase Catalytic Subunit 1
Pdpc 1
Database Links Reactome: R-HSA-204174
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase Acetyl-Transferring-Phosphatase 1 - Mitochondrial antibody
Anti-Pdp 1 antibody
Anti-Protein Phosphatase 2c antibody
Anti-Pyruvate Dehydrogenase Phosphatase Catalytic Subunit 1 antibody
Anti-Pdpc 1 antibody
Anti-PDP1 antibody
Anti-PDP antibody
Anti-PPM2C antibody

Information sourced from Uniprot.org

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