| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PDK4 (312-411) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PDK4 |
| Gene ID: |
5166 |
| Uniprot ID: |
PDK4_HUMAN |
| Immunogen Region: |
312-411 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 312-411 of human PDK4 (NP_002603.1). |
| Immunogen Sequence: |
STAPTPVMDNSRNAPLAGFG YGLPISRLYAKYFQGDLNLY SLSGYGTDAIIYLKALSSES IEKLPVFNKSAFKHYQMSSE ADDWCIPSREPKNLAKEVAM |
| Tissue Specificity | Ubiquitous.highest levels of expression in heart and skeletal muscle. |
| Function | Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism. |
| Protein Name | Pyruvate DehydrogenaseAcetyl-Transferring Kinase Isozyme 4 - MitochondrialPyruvate Dehydrogenase Kinase Isoform 4 |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase antibodyAnti-Acetyl-Transferring Kinase Isozyme 4 - Mitochondrial antibodyAnti-Pyruvate Dehydrogenase Kinase Isoform 4 antibodyAnti-PDK4 antibodyAnti-PDHK4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
