• Western blot analysis of various lysates, using PDCD10 rabbit polyclonal antibody (STJ119307) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using PDCD10 antibody (STJ119307) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using PDCD10 antibody (STJ119307) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using PDCD10 antibody (STJ119307) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of PC-12 cells using PDCD10 rabbit polyclonal antibody (STJ119307) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDCD10 antibody (1-212) (STJ119307)

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STJ119307

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDCD10 (1-212) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDCD10
Gene ID: 11235
Uniprot ID: PDC10_HUMAN
Immunogen Region: 1-212
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human PDCD10 (NP_009148.2).
Immunogen Sequence: MRMTMEEMKNEAETTSMVSM PLYAVMYPVFNELERVNLSA AQTLRAAFIKAEKENPGLTQ DIIMKILEKKSVEVNFTESL LRMAADDVEEYMIERPEPEF QDLNEKARALKQILSKIPDE INDRVRFLQTIKDIASAIKE LLDTVNNVFKKYQYQNRRAL EHQKKEFVKYSKSFSDTLKT YFKDGKAINVFVSANRLIHQ TNLILQTFKTVA
Tissue Specificity Ubiquitous.
Function Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and STK26 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development.
Protein Name Programmed Cell Death Protein 10
Cerebral Cavernous Malformations 3 Protein
Tf-1 Cell Apoptosis-Related Protein 15
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Membrane
Partially Co-Localizes With Endogenous Pxn At The Leading Edges Of Migrating Cells
Alternative Antibody Names Anti-Programmed Cell Death Protein 10 antibody
Anti-Cerebral Cavernous Malformations 3 Protein antibody
Anti-Tf-1 Cell Apoptosis-Related Protein 15 antibody
Anti-PDCD10 antibody
Anti-CCM3 antibody
Anti-TFAR15 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance