• Western blot analysis of extracts of various cell lines, using PEPCK/PEPCK/PCK2 rabbit monoclonal antibody (STJ11102086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts from wild type (WT) and PEPCK/PCK2 knockout (KO) 293T cells, using PEPCK/PCK2 antibody (STJ11102086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of HeLa cells using PEPCK/PEPCK/PCK2 rabbit monoclonal antibody (STJ11102086) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PCK2 antibody (550-640) [S6MR] (STJ11102086)

SKU:
STJ11102086

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PCK2 (550-640) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCK2
Gene ID: 5106
Uniprot ID: PCKGM_HUMAN
Immunogen Region: 550-640
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 550-640 of human PEPCK/PEPCK/PCK2 (Q16822).
Immunogen Sequence: ENARVLDWICRRLEGEDSAR ETPIGLVPKEGALDLSGLRA IDTTQLFSLPKDFWEQEVRD IRSYLTEQVNQDLPKEVLAE LEALERRVHKM
Function Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Protein Name Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial
Pepck-M
Database Links Reactome: R-HSA-70263
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial antibody
Anti-Pepck-M antibody
Anti-PCK2 antibody
Anti-PEPCK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance