| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Protein mono-ADP-ribosyltransferase PARP9 (450-530 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PARP9 |
| Gene ID: | 83666 |
| Uniprot ID: | PARP9_HUMAN |
| Immunogen Region: | 450-530 aa |
| Specificity: | PARP9 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 450-530 |
| Post Translational Modifications | ADP-ribosylated by PARP14. |
| Function | ADP-ribosyltransferase which, in association with E3 ligase DTX3L, plays a role in DNA damage repair and in immune responses including interferon-mediated antiviral defenses. Within the complex, enhances DTX3L E3 ligase activity which is further enhanced by PARP9 binding to poly(ADP-ribose). In association with DTX3L and in presence of E1 and E2 enzymes, mediates NAD(+)-dependent mono-ADP-ribosylation of ubiquitin which prevents ubiquitin conjugation to substrates such as histones. During DNA repair, PARP1 recruits PARP9/BAL1-DTX3L complex to DNA damage sites via PARP9 binding to ribosylated PARP1. Subsequent PARP1-dependent PARP9/BAL1-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. In response to DNA damage, PARP9-DTX3L complex is required for efficient non-homologous end joining (NHEJ).the complex function is negatively modulated by PARP9 activity. Dispensable for B-cell receptor (BCR) assembly through V(D)J recombination and class switch recombination (CSR). In macrophages, positively regulates pro-inflammatory cytokines production in response to IFNG stimulation by suppressing PARP14-mediated STAT1 ADP-ribosylation and thus promoting STAT1 phosphorylation. Also suppresses PARP14-mediated STAT6 ADP-ribosylation. |
| Protein Name | Protein Mono-Adp-Ribosyltransferase Parp9 Adp-Ribosyltransferase Diphtheria Toxin-Like 9 Artd9 B Aggressive Lymphoma Protein Poly Adp-Ribose Polymerase 9 Parp-9 |
| Database Links | Reactome: R-HSA-197264 Reactome: R-HSA-9683610 Reactome: R-HSA-9694631 |
| Cellular Localisation | Cytoplasm Cytosol Nucleus Shuttles Between The Nucleus And The Cytosol Translocates To The Nucleus In Response To Ifng Or Ifnb1 Stimulation Export To The Cytosol Depends On The Interaction With Dtx3l Localizes At Sites Of Dna Damage In A Parp1-Dependent Manner |
| Alternative Antibody Names | Anti-Protein Mono-Adp-Ribosyltransferase Parp9 antibody Anti-Adp-Ribosyltransferase Diphtheria Toxin-Like 9 antibody Anti-Artd9 antibody Anti-B Aggressive Lymphoma Protein antibody Anti-Poly Adp-Ribose Polymerase 9 antibody Anti-Parp-9 antibody Anti-PARP9 antibody Anti-BAL antibody Anti-BAL1 antibody |
Information sourced from Uniprot.org