• Human infiltrating breast carcinoma stained with anti-pankeratin AE1/AE3 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

Anti-Pan-Keratin antibody [AE1/AE3] (STJ180154)

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STJ180154

£152.50 - £434.50
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Host: Mouse
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Pan-Keratin is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: AE1/AE3
Conjugation: Unconjugated
Isotype: IgG1/Kappa + IgG2a/Kappa
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:100‐200
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Specificity: Positive control: Squamous cell carcinoma, prostate
Immunogen: Human epidermal keratin
Background Twenty human cytokeratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50 NA, 48, 46, 45, and 40kDa. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56 and 52kDa. Members of acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with cell type, differentiation status and environment. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. MAb AE1 recognizes the 56.5, 50, 50 NA, 48, and 40kDa keratins of acidic subfamily, whereas the AE3 MAb reacts with the basic keratins of 65-67, 64, 59, 58, 56, and 52kDa. AE1/AE3 reacts with keratinized (56.5/65-67) and corneal (55/64) epidermis, stratified squamous epithelia of internal organs (51/59) , stratified epithelia (50/58) , hyperproliferative keratinocytes (48/56) , and simple epithelia (45/52 and 46/54). AE1/AE3 is a broad-spectrum anti pan-keratin antibody cocktail which differentiates epithelial from non-epithelial tumors.

Information sourced from Uniprot.org

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