• Western blot analysis of extracts of U-87MG cells, using P3H4 antibody (STJ115702) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-P3H4 antibody (338-437) (STJ115702)

SKU:
STJ115702

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-P3H4 (338-437) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: P3H4
Gene ID: 10609
Uniprot ID: SC65_HUMAN
Immunogen Region: 338-437
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 338-437 of human P3H4 (NP_006446.1).
Immunogen Sequence: FHRARWGLEEEDFQPREEAM LYHNQTAELRELLEFTHMYL QSDDEMELEETEPPLEPEDA LSDAEFEGEGDYEEGMYADW WQEPDAKGDEAEAEPEPELA
Tissue Specificity Detected in fibroblasts (at protein level). Detected in spleen, prostate, testis, ovary, colon, pancreas, kidney, placenta and heart.
Function Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils. Required for normal bone density and normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly.
Protein Name Endoplasmic Reticulum Protein Sc65
Leprecan-Like Protein 4
Nucleolar Autoantigen No55
Prolyl 3-Hydroxylase Family Member 4
Synaptonemal Complex Protein Sc65
Cellular Localisation Endoplasmic Reticulum
Alternative Antibody Names Anti-Endoplasmic Reticulum Protein Sc65 antibody
Anti-Leprecan-Like Protein 4 antibody
Anti-Nucleolar Autoantigen No55 antibody
Anti-Prolyl 3-Hydroxylase Family Member 4 antibody
Anti-Synaptonemal Complex Protein Sc65 antibody
Anti-P3H4 antibody
Anti-LEPREL4 antibody
Anti-NOL55 antibody
Anti-SC65 antibody

Information sourced from Uniprot.org

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