| Host: | Rabbit |
| Applications: | IHC/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal antibody anti-p21 is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality : | Monoclonal |
| Clone ID : | SR0522 |
| Conjugation: | Unconjugated |
| Formulation: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range: | IHC/IF (H, M, R) 1:200-1:600 |
| Storage Instruction: | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
| Gene Symbol: | CDKN1A |
| Gene ID: | 1026 |
| Uniprot ID: | CDN1A_HUMAN |
| Post Translational Modifications | Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. Ubiquitinated by MKRN1.leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114.leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1. |
| Function | Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes. |
| Protein Name | Cyclin-Dependent Kinase Inhibitor 1 Cdk-Interacting Protein 1 Melanoma Differentiation-Associated Protein 6 Mda-6 P21 |
| Database Links | Reactome: R-HSA-187577 Reactome: R-HSA-198323 Reactome: R-HSA-2559582 Reactome: R-HSA-2559586 Reactome: R-HSA-5674400 Reactome: R-HSA-6785807 Reactome: R-HSA-6804116 Reactome: R-HSA-69202 Reactome: R-HSA-69231 Reactome: R-HSA-69563 Reactome: R-HSA-69656 Reactome: R-HSA-69895 Reactome: R-HSA-8852276 Reactome: R-HSA-8866911 Reactome: R-HSA-8878166 Reactome: R-HSA-8941855 Reactome: R-HSA-8951664 Reactome: R-HSA-9616222 Reactome: R-HSA-9617828 Reactome: R-HSA-9661069 Reactome: R-HSA-9702518 Reactome: R-HSA-9703465 Reactome: R-HSA-9725370 Reactome: R-HSA-9755511 Reactome: R-HSA-9825892 |
| Cellular Localisation | Cytoplasm Nucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase Inhibitor 1 antibody Anti-Cdk-Interacting Protein 1 antibody Anti-Melanoma Differentiation-Associated Protein 6 antibody Anti-Mda-6 antibody Anti-P21 antibody Anti-CDKN1A antibody Anti-CAP20 antibody Anti-CDKN1 antibody Anti-CIP1 antibody Anti-MDA6 antibody Anti-PIC1 antibody Anti-SDI1 antibody Anti-WAF1 antibody |
Information sourced from Uniprot.org