Western blot analysis of extracts of various cell lines, using OPRD1 antibody (STJ116139) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-OPRD1 antibody (283-372) (STJ116139)

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STJ116139
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OPRD1
Gene ID: 4985
Uniprot ID: OPRD_HUMAN
Immunogen Region: 283-372
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 283-372 of human OPRD1 (NP_000902.3).
Immunogen Sequence: VWTLVDIDRRDPLVVAALHL CIALGYANSSLNPVLYAFLD ENFKRCFRQLCRKPCGRPDP SSFSRAREATARERVTACTP SDGPGGGAAA
Tissue Specificity Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes.
Post Translational Modifications N-glycosylated. Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation.the ubiquitination is diminished by RTP4.
Function G-protein coupled receptor that functions as a receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.
Protein Name Delta-Type Opioid Receptor
D-Or-1
Dor-1
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-6785807
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Delta-Type Opioid Receptor antibody
Anti-D-Or-1 antibody
Anti-Dor-1 antibody
Anti-OPRD1 antibody
Anti-OPRD antibody

Information sourced from Uniprot.org