| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-OFD1 (763-1012) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
OFD1 |
| Gene ID: |
8481 |
| Uniprot ID: |
OFD1_HUMAN |
| Immunogen Region: |
763-1012 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 763-1012 of human OFD1 (NP_003602.1). |
| Immunogen Sequence: |
PSPCPDRMPLPSPTESRHSL SIPPVSSPPEQKVGLYRRQT ELQDKSEFSDVDKLAFKDNE EFESSFESAGNMPRQLEMGG LSPAGDMSHVDAAAAAVPLS YQHPSVDQKQIEEQKEEEKI REQQVKERRQREERRQSNLQ EVLERERRELEKLYQERKMI EESLKIKIKKELEMENELEM SNQEIKDKSAHSENPLEKYM KIIQQEQDQESADKSSKKMV QEGSLVDTLQSSDKVESLT |
| Tissue Specificity | Widely expressed. Expressed in 9 and 14 weeks old embryos in metanephric mesenchyme, oral mucosa, lung, heart, nasal and cranial cartilage, and brain. Expressed in metanephros, brain, tongue, and limb. |
| Post Translational Modifications | Phosphorylated. Phosphorylation at Ser-735, by the cAMP-dependent protein kinase PKA, triggers ubiquitination and proteasomal degradation of OFD1. Also increases its interaction with TBC1D31 and regulates its function in ciliogenesis. Ubiquitinated by PJA2, upon phosphorylation at Ser-735 by PKA, leads to the proteasomal degradation of OFD1. |
| Function | Component of the centrioles controlling mother and daughter centrioles length. Recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. The cilium is a cell surface projection found in many vertebrate cells required to transduce signals important for development and tissue homeostasis. Plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. Only OFD1 localized at the centriolar satellites is removed by autophagy, which is an important step in the ciliogenesis regulation. |
| Protein Name | Centriole And Centriolar Satellite Protein Ofd1Oral-Facial-Digital Syndrome 1 ProteinProtein 71-7a |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5610787Reactome: R-HSA-5620912Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCilium Basal BodyNucleusCentriolar SatelliteLocalizes To Centriole Distal Ends And To Centriolar SatellitesLocalization To Centrioles And Pericentriolar Satellites May Be Mediated By Kiaa0753/Ofip |
| Alternative Antibody Names | Anti-Centriole And Centriolar Satellite Protein Ofd1 antibodyAnti-Oral-Facial-Digital Syndrome 1 Protein antibodyAnti-Protein 71-7a antibodyAnti-OFD1 antibodyAnti-CXorf5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
