• Western blot analysis of extracts of various cell lines, using NUTF2 antibody (STJ29137) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NUTF2 antibody (1-127) (STJ29137)

SKU:
STJ29137

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUTF2
Gene ID: 10204
Uniprot ID: NTF2_HUMAN
Immunogen Region: 1-127
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human NUTF2 (NP_005787.1).
Immunogen Sequence: MGDKPIWEQIGSSFIQHYYQ LFDNDRTQLGAIYIDASCLT WEGQQFQGKAAIVEKLSSLP FQKIQHSITAQDHQPTPDSC IISMVVGQLKADEDPIMGFH QMFLLKNINDAWVCTNDMFR LALHNFG
Function Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.
Protein Name Nuclear Transport Factor 2
Ntf-2
Placental Protein 15
Pp15
Cellular Localisation Cytoplasm
Cytosol
Nucleus Outer Membrane
Nucleus
Nuclear Pore Complex
Nucleus Inner Membrane
Nucleoplasm
At Steady State It Is Essentially Nucleoplasmic
Enriched In Nucleoplasmic Foci
Alternative Antibody Names Anti-Nuclear Transport Factor 2 antibody
Anti-Ntf-2 antibody
Anti-Placental Protein 15 antibody
Anti-Pp15 antibody
Anti-NUTF2 antibody
Anti-NTF2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance